Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics

被引：2811

作者：

Fagerberg, Linn ^{[1
]}

Hallstrom, Bjorn M. ^{[1
]}

Oksvold, Per ^{[1
]}

Kampf, Caroline ^{[2
]}

Djureinovic, Dijana ^{[2
]}

Odeberg, Jacob ^{[1
]}

Habuka, Masato ^{[1
]}

Tahmasebpoor, Simin ^{[2
]}

Danielsson, Angelika ^{[2
]}

Edlund, Karolina ^{[2
]}

Asplund, Anna ^{[2
]}

Sjostedt, Evelina ^{[2
]}

Lundberg, Emma ^{[1
]}

Szigyarto, Cristina Al-Khalili ^{[1
]}

Skogs, Marie ^{[1
]}

Takanen, Jenny Ottosson ^{[4
]}

Berling, Holger ^{[4
]}

Tegel, Hanna ^{[4
]}

Mulder, Jan ^{[6
]}

Nilsson, Peter ^{[1
]}

Schwenk, Jochen M. ^{[1
]}

Lindskog, Cecilia ^{[2
]}

Danielsson, Frida ^{[1
]}

Mardinoglu, Adil ^{[3
]}

Sivertsson, Asa ^{[1
]}

von Feilitzen, Kalle ^{[4
]}

Forsberg, Mattias ^{[1
]}

Zwahlen, Martin ^{[1
]}

Olsson, IngMarie ^{[2
]}

Navani, Sanjay ^{[5
]}

Huss, Mikael ^{[1
]}

Nielsen, Jens ^{[1
,3
]}

Ponten, Fredrik ^{[2
]}

Uhlen, Mathias ^{[1
,4
]}

机构：

[1] KTH Royal Inst Technol, Sci Life Lab, SE-17121 Stockholm, Sweden

[2] Uppsala Univ, Dept Immunol Genet & Pathol, Rudbeck Lab, SE-75185 Uppsala, Sweden

[3] Chalmers, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden

[4] KTH Royal Inst Technol, Dept Prote, SE-10691 Stockholm, Sweden

[5] Lab Surgpath, Bombay, Maharashtra, India

[6] Karolinska Inst, Sci Life Lab, Dept Neurosci, SE-17177 Stockholm, Sweden

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2014年 / 13卷 / 02期

关键词：

HUMAN PROTEIN ATLAS; RNA-SEQ; TOOL; PLATFORM; PROJECT; CELLS;

D O I：

10.1074/mcp.M113.035600

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody-based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to approximate to 80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.

引用

页码：397 / 406

页数：10

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