Solid-phase synthesis of branched RNA and branched DNA/RNA chimeras

被引：27

作者：

Grotli, M ^{[1
]}

Eritja, R ^{[1
]}

Sproat, B ^{[1
]}

机构：

[1] INNOVIR, D-37124 ROSDORF, GERMANY

来源：

TETRAHEDRON | 1997年 / 53卷 / 33期

关键词：

D O I：

10.1016/S0040-4020(97)00731-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

An effective method for synthesising branched oligonucleotides on solid phase in the 5' to 3' direction has been developed. Special branch-point monomers enable the synthesis of branched oligonucleotides which can have sequences of different length and base composition attached to the 2'-and 3'-hydroxyl groups of the branch point ribonucleoside. The branched oligonucleotides are assembled on commercial DNA synthesisers, the crude products are readily purified by reversed phase HPLC and the fully deprotected products are conveniently analysed by mass spectrometry. (C) 1997 Elsevier Science Ltd.

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页码：11317 / 11346

页数：30

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