Microbial production of extra-cellular phytase using polystyrene as inert solid support

被引:67
作者
Gautam, P
Sabu, A
Pandey, A [1 ]
Szakacs, G
Soccol, CR
机构
[1] CSIR, Reg Res Lab, Div Biotechnol, Trivandrum 695019, Kerala, India
[2] Tech Univ Budapest, Dept Agr Chem Technol, H-1111 Budapest, Hungary
[3] Univ Fed Parana, Dept Engn Quim, Lab Proc Biotechnol, BR-81531970 Curitiba, Parana, Brazil
关键词
D O I
10.1016/S0960-8524(01)00215-2
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Aspergillus ficuum TUB F-1165 and Rhizopus oligosporus TUB F-1166 produced extra-cellular phytase during solid-state fermentation (SSF) using polystyrene as inert Support. Maximal enzyme production (10.07 U/g dry substrate (U/gds) for A. ficuum and 4.52 U/gds for R. oligosporus) was observed when SSF was carried out with substrate pH 6.0 and moisture 58.3%, incubation temperature 30 degreesC inoculum size of 1.3 x 10(7) spores/5 g substrate, for 72 h for A. ficuum and with substrate pH 7.0 and moisture 58.3%, incubation temperature 30 degreesC inoculum size of 1 x 10(6) spores/5 g substrate for 96 h for R. oligosporus. Results indicated scope for production of phytase using polystyrene as inert support. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:229 / 233
页数:5
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