Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia

被引：96

作者：

Woo, PCY ^{[1
]}

Lau, SKP ^{[1
]}

Wong, BHL ^{[1
]}

Tsoi, H ^{[1
]}

Fung, AMY ^{[1
]}

Chan, KH ^{[1
]}

Tam, VKP ^{[1
]}

Peiris, JSM ^{[1
]}

Yuen, K ^{[1
]}

机构：

[1] Univ Hong Kong, Queen Mary Hosp, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 05期

关键词：

D O I：

10.1128/JCM.42.5.2306-2309.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.

引用

页码：2306 / 2309

页数：4

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