HPLC detection of soluble carbohydrates involved in mannitol and trehalose metabolism in the edible mushroom Agaricus bisporus

被引：37

作者：

Wannet, WJB ^{[1
]}

Hermans, JHM ^{[1
]}

van der Drift, C ^{[1
]}

den Camp, HJMO ^{[1
]}

机构：

[1] Catholic Univ Nijmegen, Fac Sci, Dept Microbiol, NL-6525 ED Nijmegen, Netherlands

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 02期

关键词：

HPLC method; mannitol; trehalose; edible mushroom; Agaricus bisporus;

D O I：

10.1021/jf990596d

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A convenient and sensitive method was developed to separate and detect various types of carbohydrates (polyols, mono- and disaccharides, and phosphorylated sugars) simultaneously using high-performance liquid chromatography (HPLC). The method consists of a chromatographic separation on a CarboPac PA1 anion-exchange analytical column followed by pulsed amperometric detection. In a single run (43 min) 13 carbohydrates were readily resolved. Calibration plots were linear over the ranges of 5-25 mu M to 1.0-1.5 mM. The reliable and fast analysis technique, avoiding derivatization steps and long run times, was used to determine the levels of carbohydrates involved in mannitol and trehalose metabolism in the edible mushroom Agaricus bisporus. Moreover, the method was used to study the trehalose phosphorylase reaction.

引用

页码：287 / 291

页数：5

共 25 条

[1] POLYOL ACCUMULATION BY 2 FILAMENTOUS FUNGI GROWN AT DIFFERENT CONCENTRATIONS OF NACL [J].

ADLER, L ;

PEDERSEN, A ;

TUNBLADJOHANSSON, I .

PHYSIOLOGIA PLANTARUM, 1982, 56 (02) :139-142

[2] EFFECTS OF WATER POTENTIAL ON ACCUMULATION AND EXUDATION OF CARBOHYDRATES AND GLYCEROL DURING SCLEROTIUM FORMATION AND MYCELIOGENIC GERMINATION IN SCLEROTINIA-SCLEROTIORUM [J].

ALHAMDANI, AM ;

COOKE, RC .

TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1987, 89 :51-60

[3] MICROBIAL TRANSFORMATION OF SUCROSE AND GLUCOSE TO ERYTHRITOL [J].

AOKI, MAY ;

PASTORE, GM ;

PARK, YK .

BIOTECHNOLOGY LETTERS, 1993, 15 (04) :383-388

[4]

BAARS JJP, 1996, THESIS U NIJMEGEN NE

[5]

Beck E., 1990, Methods in Plant Biochemistry, Vol. 2: Carbohydrates., P235

[6]

BHAJEKAR DV, 1992, NAHRUNG, V36, P394, DOI 10.1002/food.19920360411

[7] CARBOHYDRATE STORAGE IN THE ENTOMOPATHOGENIC FUNGUS BEAUVERIA-BASSIANA [J].

BIDOCHKA, MJ ;

LOW, NH ;

KHACHATOURIANS, GG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (10) :3186-3190

[8]

BIELESKI RL, 1982, ENCY PLANT PHYSL A, V13, P158

[9]

Blumenthal H. J., 1976, FILAMENTOUS FUNGI, P292

[10] A rapid HPLC protocol for detection of polyols and trehalose [J].

Hallsworth, JE ;

Magan, N .

JOURNAL OF MICROBIOLOGICAL METHODS, 1997, 29 (01) :7-13

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