Highly dense protein layers confirmed by atomic force microscopy and quartz crystal microbalance

被引：24

作者：

Kim, J ^{[1
]}

Yamasaki, R ^{[1
]}

Park, J ^{[1
]}

Jung, H ^{[1
]}

Lee, H ^{[1
]}

Kawai, T ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Ibaraki, Osaka 5670047, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2004年 / 97卷 / 02期

关键词：

atomic force microscopy (AFM); quartz crystal microbalance (QCM); streptavidin; protein film;

D O I：

10.1016/S1389-1723(04)70182-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein adsorption on a gold surface is investigated by comparing the results of quartz crystal microbalance method and atomic force microscopy. The adsorption of streptavidin on functional gold surfaces is directly monitored by a quartz crystal microbalance, and confirmed by atomic force microscopy. For this investigation, a modified gold substrate is fabricated to obtain a topographic image of streptavidin molecules. Both methods show a correlation in terms of the highly dense protein single-layer formation, and the modified gold electrode shows a slightly denser protein layer formation because of the difference in substrate geometry as compared with that of a mica surface.

引用

页码：138 / 140

页数：3

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