Rolling Circle Translation of Circular RNA in Living Human Cells

被引：524

作者：

Abe, Naoko ^{[1
,2
,3
]}

Matsumoto, Ken ^{[5
]}

Nishihara, Mizuki ^{[3
]}

Nakano, Yukiko ^{[2
,3
]}

Shibata, Aya ^{[3
]}

Maruyama, Hideto ^{[2
,3
]}

Shuto, Satoshi ^{[2
]}

Matsuda, Akira ^{[2
]}

Yoshida, Minoru ^{[5
]}

Ito, Yoshihiro ^{[3
,6
]}

Abe, Hiroshi ^{[1
,2
,3
,4
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Dept Chem, Chikusa Ku, Nagoya, Aichi 4648602, Japan

[2] Hokkaido Univ, Fac Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan

[3] RIKEN, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

[4] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

[5] RIKEN, Chem Genet Lab, Wako, Saitama 3510198, Japan

[6] RIKEN, Ctr Emergent Matter Sci, Emergent Bioengn Mat Res Team, Wako, Saitama 3510198, Japan

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

MESSENGER-RNA; INITIATION; OLIGONUCLEOTIDES; AMPLIFICATION; PROKARYOTES;

D O I：

10.1038/srep16435

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

We recently reported that circular RNA is efficiently translated by a rolling circle amplification (RCA) mechanism in a cell-free Escherichia coli translation system. Recent studies have shown that circular RNAs composed of exonic sequences are abundant in human cells. However, whether these circular RNAs can be translated into proteins within cells remains unclear. In this study, we prepared circular RNAs with an infinite open reading frame and tested their translation in eukaryotic systems. Circular RNAs were translated into long proteins in rabbit reticulocyte lysate in the absence of any particular element for internal ribosome entry, a poly-A tail, or a cap structure. The translation systems in eukaryote can accept much simpler RNA as a template for protein synthesis by cyclisation. Here, we demonstrated that the circular RNA is efficiently translated in living human cells to produce abundant protein product by RCA mechanism. These findings suggest that translation of exonic circular RNAs present in human cells is more probable than previously thought.

引用

页数：9

共 35 条

[1]

Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA [J].