Different strains of mice present distinct lung tissue mechanics and extracellular matrix composition in a model of chronic allergic asthma

被引：24

作者：

Antunes, Mariana A. ^{[1
]}

Abreu, Soraia C. ^{[1
]}

Damaceno-Rodrigues, Nilsa R. ^{[2
]}

Parra, Edwin R. ^{[2
]}

Capelozzi, Vera L. ^{[2
]}

Pinart, Mariona ^{[3
]}

Romero, Pablo V. ^{[3
]}

Silva, Patricia M. R. ^{[4
]}

Martins, Marco Aurelio ^{[4
]}

Rocco, Patricia R. M. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Lab Pulm Invest, Carlos Chagas Filho Biophys Inst, Ctr Ciencias Saude,Ilha Fundao, BR-21949900 Rio De Janeiro, Brazil

[2] Univ Sao Paulo, Dept Pathol, Sao Paulo, Brazil

[3] IDIBELL Hosp, Lab Expt Pneumol, Barcelona, Spain

[4] Fiocruz MS, Inst Oswaldo Cruz, Lab Inflammat, Rio De Janeiro, Brazil

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2009年 / 165卷 / 2-3期

基金：

巴西圣保罗研究基金会;

关键词：

Tissue mechanics; Asthma; Lung parenchyma; Collagen and elastic fibres; Actin; INDUCED AIRWAY HYPERRESPONSIVENESS; ACETYLCHOLINE-INDUCED CONTRACTION; DISTAL LUNG; MOUSE MODEL; INFLAMMATION; RAT; RESPONSIVENESS; STRETCH; STRIP;

D O I：

10.1016/j.resp.2008.12.003

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The impact of genetic factors on asthma is well recognized but poorly understood. We tested the hypothesis that different mouse strains present different lung tissue strip mechanics in a model of chronic allergic asthma and that these mechanical differences may be potentially related to changes of extracellular matrix composition and/or contractile elements in lung parenchyma. Oscillatory mechanics were analysed before and after acetylcholine (ACh) in C57BL/10, BALB/c, and A/J mice, subjected or not to ovalbumin sensitization and challenge. In controls, tissue elastance (E) and resistance (R), collagen and elastic fibres' content, and alpha-actin were higher in A/J compared to BALB/c mice, which, in turn, were more elevated than in C57BL/10. A similar response pattern was observed in ovalbumin-challenged animals irrespective of mouse strain. E and R augmented more in ovalbumin-challenged A/J [E: 22%, R: 18%] than C57BL/10 mice [E: 9.4%, R: 11 %] after ACh In conclusion, lung parenchyma remodelled differently yielding distinct in vitro mechanics according to mouse strain. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：202 / 207

页数：6

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