Cloning of the mouse 25-hydroxyvitamin D3-1α-hydroxylase (CYP1α) gene

被引：5

作者：

Kimmel-Jehan, C ^{[1
]}

DeLuca, HF ^{[1
]}

机构：

[1] Univ Wisconsin, Coll Agr & Life Sci, Dept Biochem, Madison, WI 53706 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2000年 / 1475卷 / 02期

关键词：

vitamin D metabolism; cytochrome P-450; vitamin D hydroxylase; CYP1; alpha; gene structure;

D O I：

10.1016/S0304-4165(00)00065-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic clone for 25-hydroxyvitamin D-3-1 alpha-hydroxylase (1 alpha-hydroxylase) was isolated from a mouse embryonic stem cell P1 genomic library. It contains nine exons spanning 4.8 kb from the transcriptional start site. All the intron insertion sites are identical to that of the human 1 alpha-hydroxylase and human vitamin D-3 25-hydroxylase genes. A polyadenylation signal AUUAAA that differs from the consensus sequence at the second residue was identified 16 bp downstream of the 3' end of the previously reported mouse cDNA. This element is the only common natural variant described. The 3' end of the gene was determined using a RACE technique. Three poly(A) addition sites were observed 12, 15 and 22 bases from the AUUAAA element. Such distances are in agreement with what is required for maturation of mammalian pre-mRNAs. This molecular cloning makes possible the generation of transgenic mice in order to further investigate the role and importance of the 25-hydroxyvitamin D-3-1 alpha-hydroxylase (CYP1 alpha). (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：109 / 113

页数：5

共 28 条

[1]

AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303

[2] Parathyroid hormone activation of the 25-hydroxyvitamin D3-1α-hydroxylase gene promoter [J].

Brenza, HL ;

Kimmel-Jehan, C ;

Jehan, F ;

Shinki, T ;

Wakino, S ;

Anazawa, H ;

Suda, T ;

DeLuca, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (04) :1387-1391

[3] CLEAVAGE SITE DETERMINANTS IN THE MAMMALIAN POLYADENYLATION SIGNAL [J].

CHEN, F ;

MACDONALD, CC ;

WILUSZ, J .

NUCLEIC ACIDS RESEARCH, 1995, 23 (14) :2614-2620

[4]

DELUCA HF, 1974, FED PROC, V33, P2211

[5] PATHOGENESIS OF HEREDITARY VITAMIN-D-DEPENDENT RICKETS - INBORN ERROR OF VITAMIN-D METABOLISM INVOLVING DEFECTIVE CONVERSION OF 25-HYDROXYVITAMIN-D TO 1ALPHA,25-DIHYDROXYVITAMIN-D [J].

FRASER, D ;

KOOH, SW ;

KIND, HP ;

HOLICK, MF ;

TANAKA, Y ;

DELUCA, HF .

NEW ENGLAND JOURNAL OF MEDICINE, 1973, 289 (16) :817-822

[6]

FROHMAN MA, 1993, METHOD ENZYMOL, V218, P340

[7] Complete structure of the human gene for the vitamin D 1α-hydroxylase, P450c1α [J].

Fu, GK ;

Portale, AA ;

Miller, WL .

DNA AND CELL BIOLOGY, 1997, 16 (12) :1499-1507

[8] Cloning of human 25-hydroxyvitamin D-1 alpha-hydroxylase and mutations causing vitamin D-dependent rickets type 1 [J].

Fu, GK ;

Lin, D ;

Zhang, MYH ;

Bikle, DD ;

Shackleton, CHL ;

Miller, WL ;

Portale, AA .

MOLECULAR ENDOCRINOLOGY, 1997, 11 (13) :1961-1970

[9] CONTROL OF 25-HYDROXYCHOLECALCIFEROL METABOLISM BY PARATHYROID GLANDS [J].

GARABEDIAN, M ;

BOYLE, IT ;

HOLICK, MF ;

DELUCA, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (07) :1673-+

[10] Current understanding of the molecular actions of vitamin D [J].

Jones, G ;

Strugnell, SA ;

DeLuca, HF .

PHYSIOLOGICAL REVIEWS, 1998, 78 (04) :1193-1231

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