NMR determination of the Pleckstrin Homology domain oligomerisation state of the protein AKT2.

被引：5

作者：

Auguin, D ^{[1
]}

Gostan, T ^{[1
]}

Delsuc, MA ^{[1
]}

Roumestand, C ^{[1
]}

机构：

[1] Fac Pharm Montpellier, INSERM, CNRS, Ctr Biochim Struct,UMR 5048, F-34093 Montpellier 5, France

来源：

COMPTES RENDUS CHIMIE | 2004年 / 7卷 / 3-4期

关键词：

AKT; N-15; relaxation; DOSY; oligomerization state;

D O I：

10.1016/j.crci.2003.10.017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The oligomerisation state of the AKT2 Pleckstrin Homology Domain (PHD) has been investigated by two different NMR methods: the determination of the correlation time of the protein by measurement of the N-15 relaxation parameters, and the measurement of its diffusion coefficient using DOSY techniques. The two methods give similar results and assess the monomeric state of the protein in the conditions used for the structural analysis. (C) 2004 Academie des sciences. Publie par Elsevier SAS. Tons droits reserves.

引用

页码：265 / 271

页数：7

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