Novel Sxra ES cell line offers hope for Y chromosome gene-targeted mice

被引：5

作者：

Simpson, EM

Johnson, KA

Shirley, BJ

Fang, GY

Bayleran, JK

Lerner, CP

机构：

[1] Univ British Columbia, Ctr Mol Med & Therapeut, British Columbia Res Inst Children & Womens Hlth, Dept Med Genet, Vancouver, BC V5Z 4H4, Canada

[2] Jackson Lab, Bar Harbor, ME 04609 USA

[3] Eastern Maine Med Ctr, Neurogenet Lab, Bangor, ME USA

来源：

GENESIS | 2002年 / 33卷 / 02期

关键词：

Sxr(a); gene targeting; Y chromosome;

D O I：

10.1002/gene.10093

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A mouse targeted for a Y Chromosome gene has not been reported. Because the Y Chromosome is present in only one copy, and most of its genes are critical for germ cell development, such a mouse would likely be infertile. Thus, we describe a new reproductive strategy to enable transmission of targeted Y Chromosome genes to subsequent generations. The strategy uses two segregating copies of Y Chromosome genes to mimic the autosomal condition. To achieve this, we developed a new embryonic stem cell line from the XYSxr(a) mouse, which carries a duplication of the gene-rich Y Chromosome short arm. Importantly, we demonstrate germ line transmission of the YSxr(a) chromosome and describe this significant new tool as a practical solution to enable reproduction in mice targeted for Y Chromosome genes.

引用

页码：62 / 66

页数：5

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