CpG methylation directly inhibits binding of the human papillomavirus type 16 E2 protein to specific DNA sequences

被引：79

作者：

Thain, A

Jenkins, O

Clarke, AR

Gaston, K

机构：

[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

[2] SMITHKLINE BEECHAM PHARMACEUT,EPSOM KT18 5XQ,SURREY,ENGLAND

来源：

JOURNAL OF VIROLOGY | 1996年 / 70卷 / 10期

关键词：

D O I：

10.1128/JVI.70.10.7233-7235.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CpG methylation of the human papillomavirus upstream regulatory region has previously been shown to reduce virus promoter activity, Here, we demonstrate that methylation of the CpG dinucleotides contained within the binding site of the human papillomavirus type 16 E2 protein has a direct effect on the interaction of this protein with DNA. Methylation of both CpG dinucleotides within the E2 site abolishes the binding of E2.

引用

收藏

页码：7233 / 7235

页数：3

相关论文

共 27 条

[1] HUMAN PAPILLOMA-VIRUS DNAS IMMORTALIZE NORMAL HUMAN MAMMARY EPITHELIAL-CELLS AND REDUCE THEIR GROWTH-FACTOR REQUIREMENTS [J].

BAND, V ;

ZAJCHOWSKI, D ;

KULESA, V ;

SAGER, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :463-467

[2] REPRESSION OF GENES BY DNA METHYLATION DEPENDS ON CPG DENSITY AND PROMOTER STRENGTH - EVIDENCE FOR INVOLVEMENT OF A METHYL-CPG BINDING-PROTEIN [J].

BOYES, J ;

BIRD, A .

EMBO JOURNAL, 1992, 11 (01) :327-333

[3] UNEVEN DISTRIBUTION OF METHYLATION SITES WITHIN THE HUMAN PAPILLOMAVIRUS 1A-GENOME - POSSIBLE RELEVANCE TO VIRAL GENE-EXPRESSION [J].

BURNETT, TS ;

SLEEMAN, JP .

NUCLEIC ACIDS RESEARCH, 1984, 12 (23) :8847-8860

[4] ANALYSIS OF THE PHYSICAL STATE OF DIFFERENT HUMAN PAPILLOMAVIRUS DNAS IN INTRAEPITHELIAL AND INVASIVE CERVICAL NEOPLASM [J].

CULLEN, AP ;

REID, R ;

CAMPION, M ;

LORINCZ, AT .

JOURNAL OF VIROLOGY, 1991, 65 (02) :606-612

[5] THE PHYSICAL STATE OF HUMAN PAPILLOMAVIRUS TYPE-16 DNA IN BENIGN AND MALIGNANT GENITAL TUMORS [J].

DURST, M ;

KLEINHEINZ, A ;

HOTZ, M ;

GISSMANN, L .

JOURNAL OF GENERAL VIROLOGY, 1985, 66 (JUL) :1515-1522

[6] ROLE OF DNA METHYLATION IN THE REGULATION OF TRANSCRIPTION [J].

EDEN, S ;

CEDAR, H .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1994, 4 (02) :255-259

[7] BINDING OF THE HUMAN PAPILLOMAVIRUS E1 ORIGIN-RECOGNITION PROTEIN IS REGULATED THROUGH COMPLEX-FORMATION WITH THE E2 ENHANCER-BINDING PROTEIN [J].

FRATTINI, MG ;

LAIMINS, LA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12398-12402

[8] MOLECULAR-STRUCTURE OF (M5DC-DG)3 - THE ROLE OF THE METHYL-GROUP ON 5-METHYL CYTOSINE IN STABILIZING Z-DNA [J].

FUJII, S ;

WANG, AHJ ;

VANDERMAREL, G ;

VANBOOM, JH ;

RICH, A .

NUCLEIC ACIDS RESEARCH, 1982, 10 (23) :7879-7892

[9] CPG METHYLATION HAS DIFFERENTIAL-EFFECTS ON THE BINDING OF YY1 AND ETS PROTEINS TO THE BIDIRECTIONAL PROMOTER OF THE SURF-1 AND SURF-2 GENES [J].

GASTON, K ;

FRIED, M .

NUCLEIC ACIDS RESEARCH, 1995, 23 (06) :901-909

[10] THE E7 GENE OF HUMAN PAPILLOMAVIRUS TYPE-16 IS SUFFICIENT FOR IMMORTALIZATION OF HUMAN EPITHELIAL-CELLS [J].

HALBERT, CL ;

DEMERS, GW ;

GALLOWAY, DA .

JOURNAL OF VIROLOGY, 1991, 65 (01) :473-478