Application of rapid-cycle real-time polymerase chain reaction for the detection of microbial pathogens: The Mayo-Roche Rapid Anthrax Test

Rapid-cycle real-time polymerase chain reaction has immediate and important implications for diagnostic testing, in the clinical microbiology laboratory. In our experience this novel testing method has outstanding performance characteristics. The sensitivities for detecting microorganisms frequently exceed standard. culture-based assays, and the time required to complete the assays is considerably shorter than that required for culture-based assays. We describe the principle of real-time polymerase chain reaction and present clinical applications, including the detection of Bacillus anthracis, the causative agent of anthrax. This latter test is commercially available. as the result of a collaborative venture between Mayo Clinic and Roche Applied Science, hence the designation The Mayo-Roche Rapid Anthrax Test.