HPLC determination of ochratoxin A in high consumption Tunisian foods

被引：42

作者：

Ghali, R. ^{[1
]}

Hmaissia-Khlifa, K. ^{[1
]}

Ghorbel, H. ^{[1
]}

Maaroufi, K. ^{[2
]}

Hedili, A. ^{[1
]}

机构：

[1] Ctr Urgent Med Aid Tunis, Lab Biol & Toxicol, Tunis 1008, Tunisia

[2] Fac Pharm Monastir, Monastir 5000, Tunisia

来源：

FOOD CONTROL | 2009年 / 20卷 / 08期

关键词：

Foods; Ochratoxin A; HPLC-FLU; Immunoaffinity; SPICES; CONFIRMATION; NEPHROPATHY; AFLATOXINS; CLEANUP; RICE;

D O I：

10.1016/j.foodcont.2008.09.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Samples (180) of high consumption food commodities from various regions of Tunisia were analysed to determine ochratoxin A contamination levels. A high performance liquid chromatography method for ochratoxin A determination was optimized. Samples were extracted with acetonitrile/water (80:20, v/v) solution and purified by immunoaffinity column. Average recoveries at 0.5 and 2 ng/g levels ranged from 84 +/- 3.1 to 94 +/- 1.2% with a between-day coefficient of variation (RSDR) of 3.8%. The method detection limit was 0.1 ng/g and ochratoxin identity was confirmed by methyl ester formation. The whole procedure was simple and fast if compared with other existing procedures. Performed analysis indicates that 45% of monitored samples were contaminated with levels ranging from 0.11 to 33.9 ng/g. The most contaminated commodities were barley, sorghum and wheat. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：716 / 720

页数：5

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