Characterization of self-assembled monolayers for biosensor applications

被引：62

作者：

Nyquist, RM ^{[1
]}

Eberhardt, AS ^{[1
]}

Silks, LA ^{[1
]}

Li, Z ^{[1
]}

Yang, X ^{[1
]}

Swanson, BI ^{[1
]}

机构：

[1] Univ Calif Los Alamos Natl Lab, Los Alamos, NM 87545 USA

来源：

LANGMUIR | 2000年 / 16卷 / 04期

关键词：

D O I：

10.1021/la990018r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mixed monolayers of thiol-terminated poly(ethylene glycol) (PEG) and thioacetyl GM1 glycolipid on Au(lll) were examined utilizing atomic force microscopy, infrared spectroscopy, and grazing incidence X-ray diffraction to determine the composition, structure, and morphology and to characterize the specific and nonspecific interactions with protein. These monolayer architectures are robust and are readily controlled to provide a network of receptor GM1 in the PEG-terminated matrix. However, we also find significant levels of nonspecific and nonnative protein binding that reader this simplistic model system unsuitable for highly sensitive biosensor device applications.

引用

页码：1793 / 1800

页数：8

共 51 条

[1] Scanning force microscopy study of patterned monolayers of alkanethiols on gold. Importance of tip-sample contact area in interpreting force modulation and friction force microscopy images [J].

Bar, G ;

Rubin, S ;

Parikh, AN ;

Swanson, BI ;

Zawodzinski, TA ;

Whangbo, MH .

LANGMUIR, 1997, 13 (03) :373-377

[2] ORIENTATION OF CHOLERA-TOXIN BOUND TO MODEL MEMBRANES [J].

CABRALLILLY, D ;

SOSINSKY, GE ;

REED, RA ;

MCDERMOTT, MR ;

SHIPLEY, GG .

BIOPHYSICAL JOURNAL, 1994, 66 (04) :935-941

[3] A 'litmus test' for molecular recognition using artificial membranes [J].

Charych, D ;

Cheng, Q ;

Reichert, A ;

Kuziemko, G ;

Stroh, M ;

Nagy, JO ;

Spevak, W ;

Stevens, RC .

CHEMISTRY & BIOLOGY, 1996, 3 (02) :113-120

[4] DIRECT COLORIMETRIC DETECTION OF A RECEPTOR-LIGAND INTERACTION BY A POLYMERIZED BILAYER ASSEMBLY [J].

CHARYCH, DH ;

NAGY, JO ;

SPEVAK, W ;

BEDNARSKI, MD .

SCIENCE, 1993, 261 (5121) :585-588

[5] A biosensor that uses ion-channel switches [J].

Cornell, BA ;

BraachMaksvytis, VLB ;

King, LG ;

Osman, PDJ ;

Raguse, B ;

Wieczorek, L ;

Pace, RJ .

NATURE, 1997, 387 (6633) :580-583

[6] SOL-GEL ENCAPSULATION METHODS FOR BIOSENSORS [J].

DAVE, BC ;

DUNN, B ;

VALENTINE, JS ;

ZINK, JI .

ANALYTICAL CHEMISTRY, 1994, 66 (22) :A1120-A1127

[7] Self-assembled monolayers of thiols on gold electrodes for bioelectrochemistry and biosensors [J].

Dong, SJ ;

Li, JH .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1997, 42 (01) :7-13

[8] Grafted poly-(ethylene glycol) on lipid surfaces inhibits protein adsorption and cell adhesion [J].

Du, H ;

Chandaroy, P ;

Hui, SW .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1326 (02) :236-248

[9] Growth kinetics in self-assembling monolayers: a unique adsorption mechanism [J].

Eberhardt, A ;

Fenter, P ;

Eisenberger, P .

SURFACE SCIENCE, 1998, 397 (1-3) :L285-L290

[10] Defects in microcontact-printed and solution-grown self-assembled monolayers [J].

Eberhardt, AS ;

Nyquist, RM ;

Parikh, AN ;

Zawodzinski, T ;

Swanson, BI .

LANGMUIR, 1999, 15 (05) :1595-1598

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