Characterization of self-assembled monolayers for biosensor applications

被引：62

作者：

Nyquist, RM ^{[1
]}

Eberhardt, AS ^{[1
]}

Silks, LA ^{[1
]}

Li, Z ^{[1
]}

Yang, X ^{[1
]}

Swanson, BI ^{[1
]}

机构：

[1] Univ Calif Los Alamos Natl Lab, Los Alamos, NM 87545 USA

来源：

LANGMUIR | 2000年 / 16卷 / 04期

关键词：

D O I：

10.1021/la990018r

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mixed monolayers of thiol-terminated poly(ethylene glycol) (PEG) and thioacetyl GM1 glycolipid on Au(lll) were examined utilizing atomic force microscopy, infrared spectroscopy, and grazing incidence X-ray diffraction to determine the composition, structure, and morphology and to characterize the specific and nonspecific interactions with protein. These monolayer architectures are robust and are readily controlled to provide a network of receptor GM1 in the PEG-terminated matrix. However, we also find significant levels of nonspecific and nonnative protein binding that reader this simplistic model system unsuitable for highly sensitive biosensor device applications.

引用

页码：1793 / 1800

页数：8

共 51 条

[11] Epitaxy and chainlength dependent strain in self-assembled monolayers [J].

Fenter, P ;

Eberhardt, A ;

Liang, KS ;

Eisenberger, P .

JOURNAL OF CHEMICAL PHYSICS, 1997, 106 (04) :1600-1608

[12] SELF-ASSEMBLY OF N-ALKYL THIOLS AS DISULFIDES ON AU(111) [J].

FENTER, P ;

EBERHARDT, A ;

EISENBERGER, P .

SCIENCE, 1994, 266 (5188) :1216-1218

[13] PHASE-BEHAVIOR OF 2-COMPONENT SELF-ASSEMBLED MONOLAYERS OF ALKANETHIOLATES ON GOLD [J].

FOLKERS, JP ;

LAIBINIS, PE ;

WHITESIDES, GM ;

DEUTCH, J .

JOURNAL OF PHYSICAL CHEMISTRY, 1994, 98 (02) :563-571

[14] KINETIC SEPARATION OF FARADAIC CURRENTS - BINARY MONOLAYERS AS MODEL SYSTEMS [J].

FORSTER, RJ ;

FAULKNER, LR .

ANALYTICAL CHEMISTRY, 1995, 67 (07) :1232-1239

[15] Interaction of gangliosides with proteins depending on oligosaccharide chain and protein surface modification [J].

Hirai, M ;

Iwase, H ;

Arai, S ;

Takizawa, T ;

Hayashi, K .

BIOPHYSICAL JOURNAL, 1998, 74 (03) :1380-1387

[16] FORMATION OF SUPPORTED PLANAR BILAYERS BY FUSION OF VESICLES TO SUPPORTED PHOSPHOLIPID MONOLAYERS [J].

KALB, E ;

FREY, S ;

TAMM, LK .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1103 (02) :307-316

[17] PATTERNING SELF-ASSEMBLED MONOLAYERS - APPLICATIONS IN MATERIALS SCIENCE [J].

KUMAR, A ;

BIEBUYCK, HA ;

WHITESIDES, GM .

LANGMUIR, 1994, 10 (05) :1498-1511

[18] BINARY LANGMUIR AND FREUNDLICH ISOTHERMS FOR IDEAL ADSORBED SOLUTIONS [J].

LEVAN, MD ;

VERMEULEN, T .

JOURNAL OF PHYSICAL CHEMISTRY, 1981, 85 (22) :3247-3250

[19]

Lin-Vien D., 1991, The Handbook of Infrared and Raman Characteristic Frequencies of Organic Molecules

[20] Adsorption of poly(ethylene glycol) amphiphiles to form coatings which inhibit protein adsorption [J].

Malmsten, M ;

VanAlstine, JM .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 177 (02) :502-512

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