Assembly of DNA nanostructures with branched tris-DNA

被引:16
作者
Kuroda, Takahiro [1 ]
Sakurai, Yoshimasa [1 ]
Suzuki, Yukinao [1 ]
Nakamura, Akiko O. [1 ]
Kuwahara, Masayasu [1 ]
Ozaki, Hiroaki [1 ]
Sawai, Hiroaki [1 ]
机构
[1] Gunma Univ, Fac Engn, Dept Appl Chem, Gunma 3768515, Japan
关键词
branched tris-DNA; DNA assembly; nanostructures; oligomerization; oligonucleotides;
D O I
10.1002/asia.200600088
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Branched tris-DNA, in which two oligonucleotides of the same sequence and one other oligonucleotide of a different sequence are connected with a rigid central linker, was prepared chemically by using a DNA synthesizer. Two branched tris-DNA molecules with complementary DNA sequences form dimer and tetramer as well as linear and spherical oligomer complexes. The complex formation was studied by UV/thermal denaturation, enzyme digestion, gel electrophoresis, and AFM imaging.
引用
收藏
页码:575 / 580
页数:6
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