Release of NO by a nitrosyl complex upon activation by the mitochondrial reducing power

被引：41

作者：

Toledo, JC

Lopes, LGD

Alves, AA

da Silva, LP

Franco, DW ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Quim Sao Carlos, Av Trabalhador Sao Carlense 400,CP 780, BR-13560970 Sao Carlos, SP, Brazil

[2] Univ Estadual Campinas, Inst Biol, BR-13083970 Campinas, SP, Brazil

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2002年 / 89卷 / 3-4期

基金：

巴西圣保罗研究基金会;

关键词：

nitrosyl; mitochondria; NADH; ruthenium complexes;

D O I：

10.1016/S0162-0134(01)00414-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reaction of trans-[Ru(NH3)(4)P(OEt)(3)NO](3+) and mitochondria was investigated through differential pulse polarography and fluorimetry. The nitrosyl complex undergoes one-electron reduction centered on the NO ligand site. The reaction between the mitochondrial reductor and trans- [Ru(NH3)(4)P(OEt)(3) NO](2+) exhibits a second order specific rate constant calculated as k=2x10(1) M-1 s(-1). The reduced species, trans- [Ru(NH3)(4)P(OEt)(3) NO](2+), quickly releases NO, yielding trans-[Ru(NH3)(4)P(OEt)(3)H2O](2+). The low toxicities of both trans- [Ru(NH3)(4)P(OEt)(3) (NO)](2+) and trans- [Ru(NH3)(4)P(OEt)(3)H2O](2+) and its ability to release NO after reductive activation in a biological medium make the nitrosyl compound a useful model of a hypotensive drug. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：267 / 271

页数：5

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