Microfluidic temperature gradient focusing

被引：261

作者：

Ross, D ^{[1
]}

Locascio, LE

机构：

[1] Natl Inst Stand & Technol, Proc Measurements Div, Gaithersburg, MD 20899 USA

[2] Natl Inst Stand & Technol, Div Analyt Chem, Gaithersburg, MD 20899 USA

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 11期

关键词：

D O I：

10.1021/ac025528w

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new technique is described for the concentration and separation of ionic species in solution within microchannels or capillaries. Concentration is achieved by balancing the electrophoretic velocity of an analyte against the bulk flow of solution in the presence of a temperature gradient. With an appropriate buffer, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity, so that the electrophoretic and bulk velocities sum to zero at a unique point, and the analyte will be focused at that point. The technique is demonstrated for a variety of analytes, including fluorescent dyes, amino acids, DNA, proteins, and particles, and is shown to be capable of greater than 10 000-fold concentration of a dilute analyte.

引用

页码：2556 / 2564

页数：9