ACRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants

被引：401

作者：

Tang, Xu ^{[1
]}

Lowder, Levi G. ^{[2
]}

Zhang, Tao ^{[3
]}

Malzahn, Aimee A. ^{[4
]}

Zheng, Xuelian ^{[1
]}

Voytas, Daniel F. ^{[5
]}

Zhong, Zhaohui ^{[1
]}

Chen, Yiyi ^{[1
]}

Ren, Qiurong ^{[1
]}

Li, Qian ^{[1
]}

Kirkland, Elida R. ^{[2
]}

Zhang, Yong ^{[1
]}

Qi, Yiping ^{[4
,6
]}

机构：

[1] Univ Elect Sci & Technol China, Ctr Informat Biol, Sch Life Sci & Technol, Dept Biotechnol, Chengdu 610054, Peoples R China

[2] East Carolina Univ, Dept Biol, Greenville, NC 27834 USA

[3] Yangzhou Univ, Minist Educ, Co Innovat Ctr Modern Prod Technol Grain Crops, Jiangsu Key Lab Crop Genet & Physiol,Key Lab Plan, Yangzhou 225009, Jiangsu, Peoples R China

[4] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA

[5] Univ Minnesota, Ctr Genome Engn, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

[6] Univ Maryland, Inst Bioscience & Biotechnol Res, Rockville, MD 20850 USA

来源：

NATURE PLANTS | 2017年 / 3卷 / 03期

基金：

美国国家科学基金会;

关键词：

TARGETED MUTAGENESIS; HUMAN-CELLS; CPF1; SPECIFICITIES; NUCLEASES; MICE; DNA;

D O I：

10.1038/nplants.2017.18

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 generated biallelic mutations at nearly 100% efficiency at four independent sites in rice To transgenic plants. Moreover, we repurposed AsCpf1 and LbCpf1 for efficient transcriptional repression in Arabidopsis, and demonstrated a more than tenfold reduction in miR159b transcription. Our data suggest promising applications of CRISPR-Cpf1 for editing plant genomes and modulating the plant transcriptome.

引用

页数：5

共 17 条

[1] TAL Effectors: Customizable Proteins for DNA Targeting [J].

Bogdanove, Adam J. ;

Voytas, Daniel F. .

SCIENCE, 2011, 333 (6051) :1843-1846

[2] Genome Engineering With Zinc-Finger Nucleases [J].

Carroll, Dana .

GENETICS, 2011, 188 (04) :773-782

[3] Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida [J].

Endo, Akira ;

Masafumi, Mikami ;

Kaya, Hidetaka ;

Toki, Seiichi .

SCIENTIFIC REPORTS, 2016, 6

[4] Self- processing of ribozyme- flanked RNAs into guide RNAs in vitro and in vivo for CRISPR- mediated genome editing [J].

Gao, Yangbin ;

Zhao, Yunde .

JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2014, 56 (04) :343-349

[5] Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins [J].

Hur, Junho K. ;

Kim, Kyoungmi ;

Been, Kyung Wook ;

Baek, Gayoung ;

Ye, Sunghyeok ;

Hur, Junseok W. ;

Ryu, Seuk-Min ;

Lee, Youn Su ;

Kim, Jin-Soo .

NATURE BIOTECHNOLOGY, 2016, 34 (08) :807-808

[6]

Kim D, 2016, NAT BIOTECHNOL, V34, P863, DOI 10.1038/nbt.3609

[7] Generation of knockout mice by Cpf1-mediated gene targeting [J].

Kim, Yongsub ;

Cheong, Seung-A ;

Lee, Jong Geol ;

Lee, Sang-Wook ;

Lee, Myeong Sup ;

Baek, In-Jeoung ;

Sung, Young Hoon .

NATURE BIOTECHNOLOGY, 2016, 34 (08) :808-810

[8] Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells [J].

Kleinstiver, Benjamin P. ;

Tsai, Shengdar Q. ;

Prew, Michelle S. ;

Nguyen, Nhu T. ;

Welch, Moira M. ;

Lopez, Jose M. ;

McCaw, Zachary R. ;

Aryee, Martin J. ;

Joung, J. Keith .

NATURE BIOTECHNOLOGY, 2016, 34 (08) :869-+

[9] Fast and accurate long-read alignment with Burrows-Wheeler transform [J].

Li, Heng ;

Durbin, Richard .

BIOINFORMATICS, 2010, 26 (05) :589-595

[10] A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation [J].

Lowder, Levi G. ;

Zhang, Dengwei ;

Baltes, Nicholas J. ;

Paul, Joseph W., III ;

Tang, Xu ;

Zheng, Xuelian ;

Voytas, Daniel F. ;

Hsieh, Tzung-Fu ;

Zhang, Yong ;

Qi, Yiping .

PLANT PHYSIOLOGY, 2015, 169 (02) :971-+

← 1 2 →