Primary structure of EPV20, a secretory glycoprotein containing a previously uncharacterized type of domain

被引:42
作者
Larsen, LB [1 ]
Ravn, P [1 ]
Boisen, A [1 ]
Berglund, L [1 ]
Petersen, TE [1 ]
机构
[1] AARHUS UNIV,PROT CHEM LAB,DK-8000 AARHUS C,DENMARK
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 243卷 / 1-2期
关键词
EPV20; protein; amino acid sequence; bovine cDNA; disulfide bonds; domain;
D O I
10.1111/j.1432-1033.1997.0437a.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A 20-kDa glycoprotein, EPV20, was isolated from bovine milk and characterized. The primary structure was determined by cDNA and protein sequencing combined with mass spectrometry. EPV20 is a 130-residue polypeptide synthesized with a 19-residue signal peptide. The function of EPV20 is unknown, but it displays 79% sequence similarity to a putative protein deduced from a human testis cDNA sequence designated HE1 (human epididymis clone 1) (Kirchhoff, C., 1992. EMBL/GeneBank/DDBJ Databases, accession number X67698). Northern blot analysis showed the bovine EPV20 to be expressed in kidney, spleen, liver and mammary gland, but remarkably not in bovine testis. The six Cys residues of EPV20 were found to be disulfide-linked in a 1-6, 2-3 and 4-5 pattern. This disulfide arrangement has been observed in other proteins, e.g. in human prostatic acid phosphatase, but the spacing between the cystines differs. Therefore, EPV20 represents a new structure among the large group of proteins containing domains with three disulfide bonds.
引用
收藏
页码:437 / 441
页数:5
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