A tomato enzyme catalyzing the phosphorylation of 3,4-dihydroxy-2-butanone

被引：10

作者：

Herz, S ^{[1
]}

Kis, K ^{[1
]}

Bacher, A ^{[1
]}

Rohdich, F ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany

来源：

PHYTOCHEMISTRY | 2002年 / 60卷 / 01期

关键词：

3,4-dihydroxy-2-butanone 4-phosphate; riboflavin; 3,4-dihydroxy-2-butanone; dihydroxyacetone; Lycopersicon esculentum; solanaceae; tomato; kinase; phosphorylation;

D O I：

10.1016/S0031-9422(02)00056-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A riboflavin biosynthesis ribB mutant of Escherichia coli deficient of 3,4-diliydroxy-2-butanone4-phosphate synthase was complemented with a cDNA library from Lycopersicon esculentum. The complementing gene was isolated and expressed in E. coli. The resulting protein was shown to specify a 62 kDa protein which phosphorylates dihydroxyacetone, both enantiomers of 3,4-dihydroxy-2-butanone, and several other aldoses and ketoses. Sequence analysis revealed homology to dihydroacetone kinases (dak) genes from plants, animals, fungi and some eubacteria. Genes with similarity to the 5' part of the dak gene from tomato were found in many other eubacteria. The physiological role of the dak gene is still incompletely known. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：3 / 11

页数：9

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