Antiflammin-2, a nonapeptide of lipocortin-1, inhibits leukocyte chemotaxis but not arachidonic acid mobilization

We have studied the effects of antiflammin-2, a fragment corresponding to amino acids 246-254 of lipocortin-1 (HDMNKVLDL), on arachidonate mobilization and metabolism and we also determined the effect of antiflammin-2 on the chemotaxis of phagocytes. Our results demonstrated that antiflammin-2 was not able to diminish significantly [H-3]arachidonic acid mobilization stimulated by 4 beta-phorbol-12-myristate 13-acetate or calcium ionophore A23187 in murine 3T6 fibroblasts or resident peritoneal macrophages. Further, antiflammin-2 had no effect on arachidonate metabolism. In contrast, a glucocorticoid such as dexamethasone reduced significantly [H-3]arachidonic acid release and arachidonate metabolism induced in both cells. This study confirms the inhibitory effect of antiflammin on leukocyte migration and suggests that it acts partly through the inhibition of leukocyte binding to endothelial cells.