Iridoid and caffeoyl phenylethanoid glycosides of the endangered carnivorous plant Pinguicula lusitanica L. (Lentibulariaceae)

被引:7
作者
Grevenstuk, Tomas [1 ]
van der Hooft, Justin J. J. [2 ]
Vervoort, Jacques [2 ,3 ]
de Waard, Pieter [3 ]
Romano, Anabela [1 ]
机构
[1] Univ Algarve, IBB, Fac Sci & Technol, P-8005139 Faro, Portugal
[2] Wageningen Univ, Biochem Lab, NL-6703 HA Wageningen, Netherlands
[3] Wageningen Univ, Wageningen NMR Ctr, NL-6703 HA Wageningen, Netherlands
关键词
Lamiales; Globularin; Verbascoside; Hyphenated techniques; LC-MS; HPLC-SPE-NMR; SPE-NMR; MARKERS; CHEMOTAXONOMY; GLUCOSIDES; HYPHENATION; VULGARIS; VERONICA; FAMILIES;
D O I
10.1016/j.bse.2009.05.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
This work reports for the first time the identification of the major compounds of Pinguicula lusitanica, an endangered carnivorous plant species, using minimal amounts of plant material. A methanol extract was prepared from in vitro cultured plantlets and analyzed by HPLC-SPE-NMR/HPLC-MS. Three iridoid and five caffeoyl phenylethanoid glycosides were identified. These groups of natural compounds were previously reported in the Lentibulariaceae family and have been used as chemotaxonomic markers in related families. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:285 / 289
页数:5
相关论文
共 24 条
[1]
Molecular systematics and phytochemistry of Rehmannia (Scrophulariaceae) [J].
Albach, Dirk C. ;
Li, Hong-Qing ;
Zhao, Nan ;
Jensen, Soren Rosendal .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2007, 35 (05) :293-300
[2]
Albert K., 2002, On-Line LC-NMR and Related Techniques
[3]
SCALBIDOSIDE AND ALBIDOSIDE, 2 IRIDOID GLYCOSIDES FROM SCUTELLARIA-ALBIDA SUBSP COLCHICA [J].
CALIS, I ;
ERSOZ, T ;
SARACOGLU, I ;
STICHER, O .
PHYTOCHEMISTRY, 1993, 32 (05) :1213-1217
[4]
Mechanism of cytotoxicity of catechols and a naphthalenediol in PC12-AC cells: the connection between extracellular autoxidation and molecular electronic structure [J].
Chichirau, A ;
Flueraru, M ;
Chepelev, LL ;
Wright, JS ;
Willmore, WG ;
Durst, T ;
Hussain, HH ;
Charron, M .
FREE RADICAL BIOLOGY AND MEDICINE, 2005, 38 (03) :344-355
[5]
Phylogenetic analysis of Pinguicula (Lentibulariaceae):: Chloroplast DNA sequences and morphology support several geographically distinct radiations [J].
Cieslak, T ;
Polepalli, JS ;
White, A ;
Müller, K ;
Borsch, T ;
Barthlott, W ;
Steiger, J ;
Marchant, A ;
Legendre, L .
AMERICAN JOURNAL OF BOTANY, 2005, 92 (10) :1723-1736
[6]
Hyphenation of solid-phase extraction with liquid chromatography and nuclear magnetic resonance:: Application of HPLC-DAD-SPE-NMR to identification of constituents of Kanahia laniflora [J].
Clarkson, C ;
Stærk, D ;
Hansen, SH ;
Jaroszewski, JW .
ANALYTICAL CHEMISTRY, 2005, 77 (11) :3547-3553
[7]
IRIDOIDS AND VERBASCOSIDE IN CALLITRICHACEAE, HIPPURIDACEAE AND LENTIBULARIACEAE [J].
DAMTOFT, S ;
JENSEN, SR ;
THORSEN, J ;
MOLGARD, P ;
OLSEN, CE .
PHYTOCHEMISTRY, 1994, 36 (04) :927-929
[8]
IRIDOID GLUCOSIDES FROM URTICULARIA-AUSTRALIS AND PINGUICULA-VULGARIS (LENTIBULARIACEAE) [J].
DAMTOFT, S ;
JENSEN, SR ;
NIELSEN, BJ .
PHYTOCHEMISTRY, 1985, 24 (10) :2281-2283
[9]
LC-NMR coupling technology: recent advancements and applications in natural products analysis [J].
Exarchou, V ;
Krucker, M ;
van Beek, TA ;
Vervoort, J ;
Gerothanassis, IP ;
Albert, K .
MAGNETIC RESONANCE IN CHEMISTRY, 2005, 43 (09) :681-687
[10]
An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant [J].
Goncalves, Sandra ;
Escapa, Ana L. ;
Grevenstuk, Tomas ;
Romano, Anabela .
PLANT CELL TISSUE AND ORGAN CULTURE, 2008, 95 (02) :239-243