Variation of variable number of tandem repeat sequences in the 3′-untransiated region of primate dopamine transporter genes that affects reporter gene expression

被引:52
作者
Inoue-Murayama, M [1 ]
Adachi, S
Mishima, N
Mitani, H
Takenaka, O
Terao, K
Hayasaka, I
Ito, S
Murayama, Y
机构
[1] Gifu Univ, Fac Agr, Gifu 5011193, Japan
[2] Kyoto Univ, Primate Res Inst, Inuyama, Aichi 4848506, Japan
[3] Natl Inst Infect Dis, Tsukuba Primate Ctr, Tsukuba, Ibaraki 3050843, Japan
[4] Sanwa Kagaku Kenkyusyo Co Ltd, Kumamoto 8693201, Japan
[5] Natl Inst Anim Hlth, Tsukuba, Ibaraki 3050856, Japan
关键词
dopamine transporter; 3-untranslated region; variable number of tandem repeat; polymorphism; gene expression; luciferase assay; nonhuman primate;
D O I
10.1016/S0304-3940(02)01125-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Genetic polymorphism has been reported in the 3'-untranslated region (3'-UTR) of the human dopamine transporter (DAT) gene and the variable number of tandem repeat (VNTR) polymorphism has been proposed to be associated with normal personal traits or psychoneurological disorders. To assess the variation of this region in nonhuman primates, we amplified the VNTR regions by the polymerase chain reaction in several species of apes and monkeys, and determined their DNA sequences. The 3'-UTR of the chimpanzee DAT gene was also polymorphic and alleles with one or two unit(s) of a 40 bp sequence were found, while all gorillas and orangutans examined had only 2-repeat allele. Cynomolgus macaques and African green monkeys shared 11- or 12-repeat and 5-repeat alleles, respectively. By performing transient transfection assay, we found that most of the VNTR sequences of nonhuman primates exhibited higher activities on reporter gene assay as compared to those of human 9-, 10- and 11-repeat sequences. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:206 / 210
页数:5
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