Tale of two integrations, transgene and T-DNA: gene targeting by homologous recombination in rice

被引：28

作者：

Iida, S ^{[1
]}

Terada, R ^{[1
]}

机构：

[1] Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2004年 / 15卷 / 02期

关键词：

D O I：

10.1016/j.copbio.2004.02.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The first successful and reproducible gene targeting by homologous recombination, without the concomitant occurrence of ectopic events, has been reported. This will be a powerful approach for the characterization of gene function in rice, an important crop and a model for other cereal species. Models have been proposed to explain gene replacement by homologous recombination, including a possible model for Agrobacterium-mediated gene targeting using a strong positive-negative selection.

引用

页码：132 / 138

页数：7

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