Amplification of rfbE and fliC genes by polymerase chain reaction for identification and detection of Salmonella serovar Enteritidis, Dublin and Gallinarum-Pullorum

被引：13

作者：

Itoh, Y

Hirose, K

Miyake, M

Khan, AQ

Hashimoto, Y

Ezaki, T

机构：

[1] Department of Microbiology, Gifu Univ. School of Medicine, Gifu

[2] Department of Microbiology, Gifu University School of Medicine, Gifu 500

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 41卷 / 10期

关键词：

rfbE gene; fliC gene; Salmonella serovar Enteritidis; Salmonella serovar Dublin; Salmonella serovar Gallinarum-Pullorum;

D O I：

10.1111/j.1348-0421.1997.tb01928.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Polymerase chain reaction (PCR) primers for O9 antigen (rfbE) and phase 1 flagellin antigen (fliC) were designed for the rapid identification and detection of Salmonella serovar Enteritidis and Dublin. The rfbE primer pairs selectively amplified the rfbE region of group O9 Salmonella serovars. The fliC primer pairs amplified the DNAs of g,m and g,p-type flagellar antigen; Salmonella serovar Enteritidis, Dublin, and Essen. However, DNA from flagellar-negative Salmonella serovar Gallinarum-Pullorum was also amplified. The sensitivity of PCR primer pairs was 10(4) CFU/assay by boiled DNA preparation and 10(2) CFU/assay by proteinase K-treated DNA preparation.

引用

页码：791 / 794

页数：4

共 30 条

[1] CLONING OF THE RFB GENE-CLUSTER OF A GROUP-C2 SALMONELLA STRAIN - COMPARISON WITH THE RFB REGIONS OF GROUP-B AND GROUP-D [J].