Liquid chromatography of polymyxin B sulphate

被引：47

作者：

Orwa, JA ^{[1
]}

Van Gerven, A ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Catholic Univ Louvain, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Analyse Geneesmiddelen, B-3000 Louvain, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 870卷 / 1-2期

关键词：

pharmaceutical analysis; polymyxins; antibiotics;

D O I：

10.1016/S0021-9673(99)00936-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase liquid chromatography method for analysis of polymyxin B sulphate is described. The method uses a YMC-Pack Pro, C-18, 5 mu m, 250x4.6 mm I.D. column maintained at 30 degrees C. The mobile phase comprises acetonitrile-sodium sulphate (0.7%, m/v)-phosphoric acid (6.8%, v/v dilution of 85%, m/m phosphoric acid)-water (22.25:50:5:22.75) at a flow-rate of 1.0 ml/min. Detection was by UV at 215 nm. The method is able to resolve polymyxin B-1, the major component, from more than thirty other components present in the complex. Robustness was evaluated by performing a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：237 / 243

页数：7

共 12 条

[1] Liquid chromatographic analysis of a formulation containing polymyxin, gramicidin and neomycin [J].

Adams, E ;

Schepers, R ;

Gathu, LW ;

Kibaya, R ;

Roets, E ;

Hoogmartens, J .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1997, 15 (04) :505-511

[2]

BARNARD J H, 1984, Analytical Proceedings, V21, P238

[3] ISOLATION AND CHARACTERIZATION OF 3 NEW POLYMYXINS IN POLYMYXIN-B AND POLYMYXIN-E BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

ELVERDAM, I ;

LARSEN, P ;

LUND, E .

JOURNAL OF CHROMATOGRAPHY, 1981, 218 (1-3) :653-661

[4] IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF CYCLIC POLYPEPTIDE ANTIBIOTICS - POLYMYXIN-B - ITS APPLICATION TO ASSAYS OF PHARMACEUTICAL FORMULATIONS [J].

FONG, GWK ;

KHO, BT .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1979, 2 (07) :957-968

[5] PREPARATIVE-SCALE SEPARATION OF POLYMYXINS WITH AN ANALYTICAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SYSTEM BY USING DISPLACEMENT CHROMATOGRAPHY [J].

KALASZ, H ;

HORVATH, C .

JOURNAL OF CHROMATOGRAPHY, 1981, 215 (1-3) :295-302

[6] ANALYTICAL AND PREPARATIVE METHODS FOR POLYMYXIN ANTIBIOTICS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A POROUS STYRENE-DIVINYLBENZENE CO-POLYMER PACKING [J].

KIMURA, Y ;

KITAMURA, H ;

ARAKI, T ;

NOGUCHI, K ;

BABA, M ;

HORI, M .

JOURNAL OF CHROMATOGRAPHY, 1981, 206 (03) :563-572

[7]

KRISTENSEN HK, 1993, J CHROMATOGR, V628, P309

[8] ISOLATION OF 2 NEW POLYMYXIN GROUP ANTIBIOTICS (STUDIES ON ANTIBIOTICS FROM GENUS-BACILLUS .20.) [J].

SHOJI, J ;

HINOO, H ;

WAKISAKA, Y ;

KOIZUMI, K ;

MAYAMA, M ;

MATSUURA, S .

JOURNAL OF ANTIBIOTICS, 1977, 30 (12) :1029-1034

[9] SEPARATION OF POLYMYXINS AND OCTAPEPTINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

TERABE, S ;

KONAKA, R ;

SHOJI, J .

JOURNAL OF CHROMATOGRAPHY, 1979, 173 (02) :313-320

[10] MICROBIOLOGICAL AND CHEMICAL-ANALYSIS OF POLYMYXIN-B AND POLYMYXIN-E (COLISTIN) SULFATES [J].

THOMAS, AH ;

THOMAS, JM ;

HOLLOWAY, I .

ANALYST, 1980, 105 (1256) :1068-&

← 1 2 →