Structure of the ribosome-bound cricket paralysis virus IRES RNA

被引:152
作者
Schueler, Martin
Connell, Sean R.
Lescoute, Aurelie
Giesebrecht, Jan
Dabrowski, Marylena
Schroeer, Birgit
Mielke, Thorsten
Penczek, Pawel A.
Westhof, Eric
Spahn, Christian M. T.
机构
[1] Charite Univ Med Berlin, Inst Med Phys & Biophys, D-10117 Berlin, Germany
[2] Univ Louis Pasteur Strasbourg 1, Architecture & React ARN, Inst Biol Mol & Cellulaire, F-67084 Strasbourg, France
[3] Max Planck Inst Mol Genet, UltraStrukturNetzwerk, D-14195 Berlin, Germany
[4] Univ Texas, Sch Med, Houston, TX 77030 USA
关键词
D O I
10.1038/nsmb1177
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Internal ribosome entry sites (IRESs) facilitate an alternative, end-independent pathway of translation initiation. A particular family of dicistroviral IRESs can assemble elongation-competent 80S ribosomal complexes in the absence of canonical initiation factors and initiator transfer RNA. We present here a cryo-EM reconstruction of a dicistroviral IRES bound to the 80S ribosome. The resolution of the cryo-EM reconstruction, in the subnanometer range, allowed the molecular structure of the complete IRES in its active, ribosome-bound state to be solved. The structure, harboring three pseudoknot-containing domains, each with a specific functional role, shows how defined elements of the IRES emerge from a compactly folded core and interact with the key ribosomal components that form the A, P and E sites, where tRNAs normally bind. Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation.
引用
收藏
页码:1092 / 1096
页数:5
相关论文
共 36 条
[1]   The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution [J].
Ban, N ;
Nissen, P ;
Hansen, J ;
Moore, PB ;
Steitz, TA .
SCIENCE, 2000, 289 (5481) :905-920
[2]   Specificity of RNA-RNA helix recognition [J].
Battle, DJ ;
Doudna, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (18) :11676-11681
[3]   Determination of the fold of the core protein of hepatitis B virus ky electron cryomicroscopy [J].
Bottcher, B ;
Wynne, SA ;
Crowther, RA .
NATURE, 1997, 386 (6620) :88-91
[4]   Electron cryomicroscopy of biological machines at subnanometer resolution [J].
Chiu, W ;
Baker, ML ;
Jiang, W ;
Dougherty, M ;
Schmid, MF .
STRUCTURE, 2005, 13 (03) :363-372
[5]   A preformed compact ribosome-binding domain in the cricket paralysis-like virus IRES RNAs [J].
Costantino, D ;
Kieft, JS .
RNA, 2005, 11 (03) :332-343
[6]   Gene-specific regulation by general translation factors [J].
Dever, TE .
CELL, 2002, 108 (04) :545-556
[7]   SPIDER and WEB: Processing and visualization of images in 3D electron microscopy and related fields [J].
Frank, J ;
Radermacher, M ;
Penczek, P ;
Zhu, J ;
Li, YH ;
Ladjadj, M ;
Leith, A .
JOURNAL OF STRUCTURAL BIOLOGY, 1996, 116 (01) :190-199
[8]   Localization and dynamic behavior of ribosomal protein L30e [J].
Halic, M ;
Becker, T ;
Frank, J ;
Spahn, CMT ;
Beckmann, R .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (05) :467-468
[9]   High resolution structure of the large ribosomal subunit from a mesophilic Eubacterium [J].
Harms, J ;
Schluenzen, F ;
Zarivach, R ;
Bashan, A ;
Gat, S ;
Agmon, I ;
Bartels, H ;
Franceschi, F ;
Yonath, A .
CELL, 2001, 107 (05) :679-688
[10]   Internal ribosome entry sites in eukaryotic mRNA molecules [J].
Hellen, CUT ;
Sarnow, P .
GENES & DEVELOPMENT, 2001, 15 (13) :1593-1612