Zoosporicidal activity of polyflavonoid tannin identified in Lannea coromandelica stem bark against phytopathogenic oomycete Aphanomyces cochlioides

被引：41

作者：

Islam, MT ^{[1
]}

Ito, T ^{[1
]}

Sakasai, M ^{[1
]}

Tahara, S ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Agr, Div Appl Geosci, Lab Ecol Chem,Kita Ku, Sapporo, Hokkaido 0608589, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 23期

关键词：

Lannea coromandelica; polyflavonoid tannins; motility-inhibiting activity; Aphanomyces cochlioides; zoospore lysis;

D O I：

10.1021/jf020554g

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

In a survey of nonhost plant secondary metabolites regulating motility and viability of zoospores of the Aphanomyces cochlioides, we found that stem bark extracts of Lannea coromandelica remarkably inhibited. motility of zoospores followed by lysis. Bioassay-guided fractionation and chemical characterization of Lannea extracts by MALDI-TOF-MS revealed that the active constituents were angular type polyflavonoid tannins. Commercial polyflavonoid tannins, Quebracho, and Mimosa, also showed identical zoosporicidal activity. Against zoospores, the motility-inhibiting and lytic activities were more pronounced in Lannea extracts (MIC 0.1 mug/mL) than in Quebracho (MIC 0.5 mug/mL) and Mimosa (MIC 0.5 mug/mL). Scanning electron microscopic observation visualized that both Lannea and commercial tannins caused lysis of cell membrane followed by fragmentation of cellular materials. Naturally occurring polyflavonoid tannin merits further study as, potential zoospore regulating agent or as lead compound. To the best of our knowledge, this is the first report of zoosporicidal activity of natural polyflavonoid tannins against an oomycete phytopathogen.

引用

页码：6697 / 6703

页数：7

共 30 条

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