Recent H3N2 Influenza Virus Clinical Isolates Rapidly Acquire Hemagglutinin or Neuraminidase Mutations When Propagated for Antigenic Analyses

被引：40

作者：

Chambers, Benjamin S. ^{[1
,2
]}

Li, Yang ^{[1
,2
]}

Hodinka, Richard L. ^{[3
,4
]}

Hensley, Scott E. ^{[1
,2
]}

机构：

[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Microbiol, Perelman Sch Med, Philadelphia, PA 19104 USA

[3] Univ Penn, Childrens Hosp Philadelphia, Clin Virol Lab, Philadelphia, PA 19104 USA

[4] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA

来源：

JOURNAL OF VIROLOGY | 2014年 / 88卷 / 18期

基金：

美国国家卫生研究院;

关键词：

A VIRUS; ANTIBODIES; RECEPTORS; EVOLUTION; VARIANTS; MUTANTS; DRIFT;

D O I：

10.1128/JVI.01077-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Prior to serological testing, influenza viruses are typically propagated in eggs or cell culture. Recent human H3N2 strains bind to cells with low avidity. Here, we isolated nine primary H3N2 viral isolates from respiratory secretions of children. Upon propagation in vitro, five of these isolates acquired hemagglutinin or neuraminidase mutations that increased virus binding to cell surfaces. These mutations can potentially confound serological assays commonly used to identify antigenically novel influenza viruses.

引用

页码：10986 / 10989

页数：4

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