Rapid recycle 13C',15N and 13C,13C' heteronuclear and homonuclear multiple quantum coherence detection for resonance assignments in paramagnetic proteins:: Example of Ni2+-containing acireductone dioxygenase

NMR resonance assignments in the vicinity of paramagnetic metals in proteins are often difficult or impossible to make using conventional 1H detected 2-D and 3-D methods due to paramagnetic line broadening. The applicability of 13Cα{13C′} and 13C′{15N} multiple quantum coherence methods for residue-specific assignments of resonances near paramagnetic centers is described, using the Ni2+-containing enzyme acireductone dioxygenase as an example. Copyright © 2002 American Chemical Society.