IL-10 alters DC function via modulation of cell surface molecules resulting in impaired T-cell responses

被引:97
作者
McBride, JM [1 ]
Jung, T [1 ]
de Vries, JE [1 ]
Aversa, G [1 ]
机构
[1] Novartis Res Inst, Autoimmune Dis Unit, A-1235 Vienna, Austria
关键词
human; dendritic cells; tolerance/suppression; lipopolysaccharide; cytokines;
D O I
10.1016/S0008-8749(02)00007-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
IL-10 is a potent inhibitor of T-cell activation and has tolerizing effects on these cells. These effects are primarily mediated via modulation of antigen presenting cell function. Here, it is demonstrated that IL-10 completely inhibits LPS-induced DC maturation, resulting in altered DC-T-cell interactions and reduced T-cell responses. IL-10 inhibited LPS-induced upregulation of costimulatory molecules, MHC Class II, and the secretion of IL-12, TNF-alpha, IL-6, and IL-1beta by DCs, although it upregulated the SLAM (CD150) expression at both the mRNA and protein levels. IL-10 pre-treated DC did not respond to subsequent LPS activation and its stimulatory ability for allogeneic and antigen-specific T-cells was severely impaired. Importantly, T-cells derived from co-cultures with Ag-pulsed, IL-10-treated DC were impaired in their responses to subsequent Ag-specific restimulation. Transwell and DC-derived plasma membrane experiments indicated that the capacity of IL-10-treated DC to induce T-cell unresponsiveness results from alterations in the cell surface molecules rather than modulation of cytokine secretion. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:162 / 172
页数:11
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