The mechanism of type IA topoisomerases

被引：112

作者：

Dekker, NH

Rybenkov, VV

Duguet, M

Crisona, NJ

Cozzarelli, NR

Bensimon, D

Croquette, V

机构：

[1] Ecole Normale Super, CNRS, UMR 8550, Lab Phys Stat, F-75231 Paris, France

[2] Ecole Normale Super, CNRS, UMR 8550, Dept Biol, F-75231 Paris, France

[3] Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA

[4] Univ Paris 11, Inst Microbiol & Genet, CNRS, UMR 8621,Lab Enzymol Acides Nucl, F-91405 Orsay, France

[5] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷 / 19期

关键词：

D O I：

10.1073/pnas.132378799

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The topology of cellular DNA is carefully controlled by enzymes called topoisomerases. By using single-molecule techniques, we monitored the activity of two type IA topoisomerases in real time under conditions in which single relaxation events were detected. The strict one-at-a-time removal of supercoils we observed establishes that these enzymes use an enzyme-bridged strand-passage mechanism that is well suited to their physiological roles and demonstrates a mechanistic unity with type II topoisomerases.

引用

页码：12126 / 12131

页数：6

共 29 条

[1] Structural similarities between topoisomerases that cleave one or both DNA strands [J].