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Multiplexed sandwich assays in microarray format

被引：142
作者：
Nielsen, UB
Geierstanger, BH
机构：
[1] Merrimack Pharmaceut, Cambridge, MA 02142 USA
[2] Novartis Res Fdn, Genomics Inst, San Diego, CA 92121 USA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2004年 / 290卷 / 1-2期
关键词：
multiplexed sandwich assays; microarray; ELISA;
D O I：
10.1016/j.jim.2004.04.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Antibody arrays have evolved into powerful tools for quantifying proteins and qualifying their state of activation in complex biological samples. This level of analysis holds tremendous promise as part of a diagnostic or prognostic platform. In particular, multiplex sandwich ELISA assays performed with microarrays in the wells of multi-well plates enable high-throughput analysis of multiple samples with multivariate readouts. Here, we compare and review recent advances in antibody microarray technology and describe its promises towards the systematic analysis of complex biological samples, ranging from profiling patient sera to studying intracellular signaling. (C) 2004 Elsevier B.V. All rights reserved.
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页码：107 / 120
页数：14
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