Fast manipulation of cellular cAMP level by light in vivo

被引：184

作者：

Schroeder-Lang, Saskia

Schwaerzel, Martin ^{[1
]}

Seifert, Reinhard

Struenker, Timo

Kateriya, Suneel

Looser, Jens

Watanabe, Masakatsu

Kaupp, U. Benjamin

Hegemann, Peter

Nagel, Georg

机构：

[1] Max Planck Inst Biophys, D-60439 Frankfurt, Germany

[2] Univ Saarland, Nat Wissensch Tech Fak 3, D-66041 Saarbrucken, Germany

[3] Forschungszentrum Julich, Inst Neurowissensch & Biophys, Abt Zellulare Signalverarbeitung, D-52425 Julich, Germany

[4] Humboldt Univ, D-10115 Berlin, Germany

[5] Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany

[6] Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan

[7] Grad Univ Adv Studies SOKENDAI, Sch Adv Sci, Hayama 2400193, Japan

来源：

NATURE METHODS | 2007年 / 4卷 / 01期

关键词：

D O I：

10.1038/NMETH975

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACa and PACb. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells. (c) 2007 Nature Publishing Group.

引用

收藏

页码：39 / 42

页数：4

相关论文

共 15 条

[1] CONTROL OF LIGAND SPECIFICITY IN CYCLIC NUCLEOTIDE-GATED CHANNELS FROM ROD PHOTORECEPTORS AND OLFACTORY EPITHELIUM [J].

ALTENHOFEN, W ;

LUDWIG, J ;

EISMANN, E ;

KRAUS, W ;

BONIGK, W ;

KAUPP, UB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9868-9872

[2] Structure of a novel photoreceptor, the BLUF domain of AppA from Rhodobacter sphaeroides [J].

Anderson, S ;

Dragnea, V ;

Masuda, S ;

Ybe, J ;

Moffat, K ;

Bauer, C .

BIOCHEMISTRY, 2005, 44 (22) :7998-8005

[3]

BRAND AH, 1993, DEVELOPMENT, V118, P401

[4] Regulation and organization of adenylyl cyclases and cAMP [J].

Cooper, DMF .

BIOCHEMICAL JOURNAL, 2003, 375 :517-529

[5] Hydrogen-bond switching through a radical pair mechanism in a flavin-binding photoreceptor [J].

Gauden, Magdalena ;

van Stokkum, Ivo H. M. ;

Key, Jason M. ;

Luehrs, Daniel Ch. ;

Van Grondelle, Rienk ;

Hegemann, Peter ;

Kennis, John T. M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (29) :10895-10900

[6] A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis [J].

Iseki, M ;

Matsunaga, S ;

Murakami, A ;

Ohno, K ;

Shiga, K ;

Yoshida, K ;

Sugai, M ;

Takahashi, T ;

Hori, T ;

Watanabe, M .

NATURE, 2002, 415 (6875) :1047-1051

[7]

Melzig J, 1998, J NEUROSCI, V18, P7160

[8] Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid Behavioral responses [J].

Nagel, G ;

Brauner, M ;

Liewald, JF ;

Adeishvili, N ;

Bamberg, E ;

Gottschalk, A .

CURRENT BIOLOGY, 2005, 15 (24) :2279-2284

[9] THE PROTEIN KINASE-A-REGULATED CARDIAC CL- CHANNEL RESEMBLES THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR [J].

NAGEL, G ;

HWANG, TC ;

NASTIUK, KL ;

NAIRN, AC ;

GADSBY, DC .

NATURE, 1992, 360 (6399) :81-84

[10] A uniform extracellular stimulus triggers distinct cAMP signals in different compartments of a simple cell [J].

Rich, TC ;

Fagan, KA ;

Tse, TE ;

Schaack, J ;

Cooper, DMF ;

Karpen, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (23) :13049-13054