Controlled Gating of Lysenin Pores

被引：18

作者：

Fologea, Daniel ^{[1
]}

Krueger, Eric ^{[2
]}

Lee, Rachel ^{[2
]}

Naglak, Matt ^{[2
]}

Mazur, Yuriy ^{[2
]}

Henry, Ralph ^{[1
]}

Salamo, Greg ^{[2
]}

机构：

[1] Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA

[2] Univ Arkansas, Dept Phys, Fayetteville, AR 72701 USA

来源：

BIOPHYSICAL CHEMISTRY | 2010年 / 146卷 / 01期

关键词：

Lysenin; Voltage gated pores; Negative conductance; BIMOLECULAR LIPID MEMBRANES; GATED ION CHANNELS; EISENIA-FOETIDA; NEGATIVE-RESISTANCE; BINDING-PROTEIN; SPHINGOMYELIN; VOLTAGE; TOXIN;

D O I：

10.1016/j.bpc.2009.09.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：25 / 29

页数：5

共 20 条

[1] Voltage-gated ion channels [J].

Bezanilla, F .

IEEE TRANSACTIONS ON NANOBIOSCIENCE, 2005, 4 (01) :34-48

[2] The voltage sensor in voltage-dependent ion channels [J].

Bezanilla, F .

PHYSIOLOGICAL REVIEWS, 2000, 80 (02) :555-592

[3] Dissection of the mechanisms of cytolytic and antibacterial activity of lysenin, a defence protein of the annelid Eisenia fetida [J].

Bruhn, Heike ;

Winkelmann, Julia ;

Andersen, Christian ;

Andrae, Joerg ;

Leippe, Matthias .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2006, 30 (07) :597-606

[4] Disturbance of smooth muscle regulatory function by Eisenia foetida toxin lysenin:: Insight into the mechanism of smooth muscle contraction [J].

Czurylo, Edward A. ;

Kulikova, Natalia ;

Sobota, Andrzej .

TOXICON, 2008, 51 (06) :1090-1102

[5] ION EFFECTS ON GATING OF THE CA2+-ACTIVATED K+ CHANNEL CORRELATE WITH OCCUPANCY OF THE PORE [J].

DEMO, SD ;

YELLEN, G .

BIOPHYSICAL JOURNAL, 1992, 61 (03) :639-648

[6] NATURE OF NEGATIVE RESISTANCE IN BIMOLECULAR LIPID MEMBRANES CONTAINING EXCITABILITY-INDUCING MATERIAL [J].

EHRENSTEIN, G ;

LECAR, H ;

NOSSAL, R .

JOURNAL OF GENERAL PHYSIOLOGY, 1970, 55 (01) :119-+

[7] INWARD RECTIFICATION IN MOUSE MACROPHAGES - EVIDENCE FOR A NEGATIVE-RESISTANCE REGION [J].

GALLIN, EK ;

LIVENGOOD, DR .

AMERICAN JOURNAL OF PHYSIOLOGY, 1981, 241 (01) :C9-C17

[8]

GUDOWSKANOWAK E, 2002, UNSOLVED PROBLEMS NO, P305

[9] Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes [J].

Ide, T ;

Aoki, T ;

Takeuchi, Y ;

Yanagida, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 346 (01) :288-292

[10] Lysenin: A new tool for investigating membrane lipid organization [J].

Ishitsuka R. ;

Kobayashi T. .

Anatomical Science International, 2004, 79 (4) :184-190

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