Thin-film polymer light emitting diodes as integrated excitation sources for microscale capillary electrophoresis

被引：60

作者：

Edel, JB ^{[1
]}

Beard, NP

Hofmann, O

DeMello, JC

Bradley, DDC

deMello, AJ

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2AZ, England

[3] Mol Vis Ltd, London EC4A 1JP, England

来源：

LAB ON A CHIP | 2004年 / 4卷 / 02期

关键词：

D O I：

10.1039/b313503a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the use of a thin-film polymer light emitting diode as an integrated excitation source for microfabricated capillary electrophoresis. The polyfluorene-based diode has a peak emission wavelength of 488 nm, an active area of 40 mum x 1000 mum and a thickness of similar to2 mm. The simple layer-by-layer deposition procedures used to fabricate the polymer component allow facile integration with planar chip-based systems. To demonstrate the efficacy of the approach, the polyfluorene diode is used as an excitation source for the detection of fluorescent dyes separated on-chip by electrophoresis. Using a conventional confocal detection system the integrated pLED is successfully used to detect fluorescein and 5-carboxyfluorescein at concentrations as low as 10(-6) M with a mass detection limit of 50 femtomoles. The drive voltages required to generate sufficient emission from the polymer diode device are as low as 3.7 V.

引用

页码：136 / 140

页数：5

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