Electrophysiological and morphological properties of neurons acutely isolated from the rostral gustatory zone of the rat nucleus of the solitary tract

被引:7
作者
Du, JH
Bradley, RM
机构
[1] UNIV MICHIGAN,SCH DENT 6228,DEPT BIOL & MAT SCI,ANN ARBOR,MI 48109
[2] UNIV MICHIGAN,SCH MED,DEPT PHYSIOL,ANN ARBOR,MI 48109
关键词
D O I
10.1093/chemse/21.6.729
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
The biophysical and morphological characteristics of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) were investigated under current clamp conditions and compared with the results obtained from neurons recorded in brain slices. The passive membrane properties of the isolated neurons were similar to rNST neurons in brain slices and the neurons maintained their morphological characteristics although their dendritic tree was truncated. The isolated neurons also retained their characteristic repetitive firing properties. In addition we also noted developmental changes in the intrinsic membrane properties of the isolated neurons, such as a shortening in action potential duration, decrease in membrane time constant and input resistance, that occurred when these parameters were compared in neurons isolated from young (5-10 days) and older animals. These enzymatically dispersed neurons therefore retained both the membrane properties and morphology observed in the intact brainstem and in vitro brain slice preparation. The use of this isolated neuron preparation provides a basis for further study of rNST neurobiology.
引用
收藏
页码:729 / 737
页数:9
相关论文
共 22 条
[1]   DEVELOPMENT OF INTRINSIC ELECTROPHYSIOLOGICAL PROPERTIES IN NEURONS FROM THE GUSTATORY REGION OF RAT NUCLEUS OF SOLITARY TRACT [J].
BAO, H ;
BRADLEY, RM ;
MISTRETTA, CM .
DEVELOPMENTAL BRAIN RESEARCH, 1995, 86 (1-2) :143-154
[2]   SEPARATION OF NEURON TYPES IN THE GUSTATORY ZONE OF THE NUCLEUS-TRACTUS-SOLITARII ON THE BASIS OF INTRINSIC FIRING PROPERTIES [J].
BRADLEY, RM ;
SWEAZEY, RD .
JOURNAL OF NEUROPHYSIOLOGY, 1992, 67 (06) :1659-1668
[3]   A GOLGI ANALYSIS OF THE GUSTATORY ZONE OF THE NUCLEUS OF THE SOLITARY TRACT IN THE ADULT HAMSTER [J].
DAVIS, BJ ;
JANG, TC .
JOURNAL OF COMPARATIVE NEUROLOGY, 1988, 278 (03) :388-396
[4]   SYNAPTIC PROCESSING OF TASTE-QUALITY INFORMATION IN NUCLEUS TRACTUS SOLITARIUS OF RAT [J].
DOETSCH, GS ;
ERICKSON, RP .
JOURNAL OF NEUROPHYSIOLOGY, 1970, 33 (04) :490-&
[5]   IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].
HAMILL, OP ;
MARTY, A ;
NEHER, E ;
SAKMANN, B ;
SIGWORTH, FJ .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100
[6]   CENTRAL PROJECTIONS OF GUSTATORY NERVES IN THE RAT [J].
HAMILTON, RB ;
NORGREN, R .
JOURNAL OF COMPARATIVE NEUROLOGY, 1984, 222 (04) :560-577
[7]   ISOLATION OF NEURONS SUITABLE FOR PATCH-CLAMPING FROM ADULT MAMMALIAN CENTRAL NERVOUS SYSTEMS [J].
KAY, AR ;
WONG, RKS .
JOURNAL OF NEUROSCIENCE METHODS, 1986, 16 (03) :227-238
[8]   DIETARY-SODIUM CHLORIDE DEPRIVATION THROUGHOUT DEVELOPMENT SELECTIVELY INFLUENCES THE TERMINAL FIELD ORGANIZATION OF GUSTATORY AFFERENT-FIBERS PROJECTING TO THE RAT NUCLEUS OF THE SOLITARY TRACT [J].
KING, CT ;
HILL, DL .
JOURNAL OF COMPARATIVE NEUROLOGY, 1991, 303 (01) :159-169
[9]   RELATIONSHIP BETWEEN STRUCTURE AND FUNCTION OF NEURONS IN THE RAT ROSTRAL NUCLEUS-TRACTUS-SOLITARII [J].
KING, MS ;
BRADLEY, RM .
JOURNAL OF COMPARATIVE NEUROLOGY, 1994, 344 (01) :50-64
[10]   POSTNATAL-DEVELOPMENT OF THE PARABRACHIAL GUSTATORY ZONE IN RAT - DENDRITIC MORPHOLOGY AND MITOCHONDRIAL ENZYME-ACTIVITY [J].
LASITER, PS ;
KACHELE, DL .
BRAIN RESEARCH BULLETIN, 1988, 21 (01) :79-94