Re-characterisation of Saccharomyces cerevisiae Ach1p: Fungal CoA-transferases are involved in acetic acid detoxification

被引:58
作者
Fleck, Christian B. [1 ]
Brock, Matthias [1 ]
机构
[1] Hans Knoell Inst, Res Grp Microbial Biochem & Physiol, Leibniz Inst Nat Prod Res & Infect Biol eV, D-07745 Jena, Germany
关键词
Acetyl-CoA hydrolase; CoA-transferase; Acetic acid detoxification; Succinyl-CoA; Physiological adaptation; ASPERGILLUS-NIDULANS; METHYLCITRATE SYNTHASE; CANDIDA-ALBICANS; ACETATE UTILIZATION; ANTIFUNGAL AGENT; GENE ENCODES; HYDROLASE; GROWTH; COENZYME; MUTANT;
D O I
10.1016/j.fgb.2009.03.004
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Saccharomyces cerevisiae and Neurospora crassa mutants defective in the so-called acetyl-CoA hydrolases Ach1p and Acu-8, respectively, display a severe growth defect on acetate, which is most strongly pronounced under acidic conditions. Acetyl-CoA hydrolysis is an energy wasting process and therefore denoted as a biochemical conundrum. Acetyl-CoA hydrolases show high sequence identity to the CoA-transferase CoaT from Aspergillus nidulans. Therefore, we extensively re-characterised the yeast enzyme. Ach1p showed highest specific activity for the CoASH transfer front succinyl-CoA to acetate and only a minor acetyl-CoA-hydrolase activity. Complementation of an ach1 mutant with the coaT gene reversed the growth defect on acetate confirming the in vivo function of Ach1p as a CoA-transferase. Our results imply that Ach1p is involved in mitochondrial acetate detoxification by a CoASH transfer from succinyl-CoA to acetate. Thereby, Ach1p does not perform the energy wasting hydrolysis of acetyl-CoA but conserves energy by the detoxification of mitochondrial acetate. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:473 / 485
页数:13
相关论文
共 33 条
[31]   Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae:: Role of the cytosolic Mg2+ and mitochondrial K+ acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation [J].
Remize, F ;
Andrieu, E ;
Dequin, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) :3151-3159
[32]  
SRERE PA, 1963, BIOCHIM BIOPHYS ACTA, V73, P523, DOI 10.1016/0006-3002(63)90458-X
[33]   COLORIMETRIC ALCOHOL ASSAYS WITH ALCOHOL OXIDASE [J].
VERDUYN, C ;
VANDIJKEN, JP ;
SCHEFFERS, WA .
JOURNAL OF MICROBIOLOGICAL METHODS, 1984, 2 (01) :15-25