Use of an automatic DNA sequencer for S1 mapping: Transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon

被引:1
作者
Brans, A [1 ]
Loriaux, A [1 ]
Thamm, I [1 ]
Joris, B [1 ]
Dusart, J [1 ]
机构
[1] UNIV LIEGE,INST CHIM,CTR INGN PROTEINES,B-4000 LIEGE 1,BELGIUM
关键词
chaperone; CIRCE motif; dnaK operon; heat shock promoter; heat shock protein;
D O I
10.1016/S0378-1097(97)00074-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by SI mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma(70)-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.
引用
收藏
页码:189 / 194
页数:6
相关论文
共 19 条
[1]   AUTOMATED DNA SEQUENCING - ULTRASENSITIVE DETECTION OF FLUORESCENT BANDS DURING ELECTROPHORESIS [J].
ANSORGE, W ;
SPROAT, B ;
STEGEMANN, J ;
SCHWAGER, C ;
ZENKE, M .
NUCLEIC ACIDS RESEARCH, 1987, 15 (11) :4593-4602
[2]   SIZING AND MAPPING OF EARLY ADENOVIRUS MESSENGER-RNAS BY GEL-ELECTROPHORESIS OF S1 ENDONUCLEASE-DIGESTED HYBRIDS [J].
BERK, AJ ;
SHARP, PA .
CELL, 1977, 12 (03) :721-732
[3]   Cloning and sequencing of the DnaK locus in Streptomyces coelicolor A3(2) [J].
Brans, A ;
Loriaux, A ;
Joris, B ;
Dusart, J .
DNA SEQUENCE, 1996, 6 (03) :179-184
[4]  
BRANS A, 1995, THESIS U LIEGE BELGI
[5]   THE DNAK OPERON OF STREPTOMYCES-COELICOLOR ENCODES A NOVEL HEAT-SHOCK PROTEIN WHICH BINDS TO THE PROMOTER REGION OF THE OPERON [J].
BUCCA, G ;
FERINA, G ;
PUGLIA, AM ;
SMITH, CP .
MOLECULAR MICROBIOLOGY, 1995, 17 (04) :663-674
[6]   CLONING AND SEQUENCING OF THE DNAK REGION OF STREPTOMYCES-COELICOLOR A3(2) [J].
BUCCA, G ;
SMITH, CP ;
ALBERTI, M ;
SEIDITA, G ;
PASSANTINO, R ;
PUGLIA, AM .
GENE, 1993, 130 (01) :141-144
[7]   TRANSCRIPTIONAL ANALYSIS OF GROEL GENES IN STREPTOMYCES-COELICOLOR A3(2) [J].
DUCHENE, AM ;
THOMPSON, CJ ;
MAZODIER, P .
MOLECULAR & GENERAL GENETICS, 1994, 245 (01) :61-68
[8]  
GUGLIELMI G, 1993, INDUSTRIAL MICROORGANISMS: BASIC AND APPLIED MOLECULAR GENETICS, P17
[9]   SEA-URCHIN HISTONE MESSENGER-RNA TERMINI ARE LOCATED IN GENE REGIONS DOWNSTREAM FROM PUTATIVE REGULATORY SEQUENCES [J].
HENTSCHEL, C ;
IRMINGER, JC ;
BUCHER, P ;
BIRNSTIEL, ML .
NATURE, 1980, 285 (5761) :147-151
[10]  
Hopwood D.A., 1985, GENETIC MANIPULATION