Use of an automatic DNA sequencer for S1 mapping: Transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon

被引：1

作者：

Brans, A ^{[1
]}

Loriaux, A ^{[1
]}

Thamm, I ^{[1
]}

Joris, B ^{[1
]}

Dusart, J ^{[1
]}

机构：

[1] UNIV LIEGE,INST CHIM,CTR INGN PROTEINES,B-4000 LIEGE 1,BELGIUM

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 149卷 / 02期

关键词：

chaperone; CIRCE motif; dnaK operon; heat shock promoter; heat shock protein;

D O I：

10.1016/S0378-1097(97)00074-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by SI mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma(70)-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.

引用

页码：189 / 194

页数：6

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