Antiproliferative and antioxidant properties of anthocyanin-rich extract from acai

被引:99
作者
Hogan, Shelly [1 ]
Chung, Hyun [1 ]
Zhang, Lei [2 ]
Li, Jianrong [2 ]
Lee, Yongwoo [3 ]
Dai, Yumin [1 ]
Zhou, Kequan [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Dept Food Sci & Technol, Blacksburg, VA 24061 USA
[2] Zhejiang Gongshang Univ, Coll Food Sci Biotechnol & Environm Engn, Hangzhou 310035, Zhejiang, Peoples R China
[3] Virginia Tech, Dept Biomed Engn & Pathobiol, Blacksburg, VA 24061 USA
关键词
Acai; Anthocyanins; Antiproliferative activity; C-6 glioma cells; Antioxidant properties; RADICAL SCAVENGING CAPACITY; AMAZONIAN PALM BERRY; OLERACEAE MART. ACAI; CANCER-CELLS; IN-VITRO; VACCINIUM-MYRTILLUS; REACTIVE OXYGEN; FRUITS; APOPTOSIS; DNA;
D O I
10.1016/j.foodchem.2009.04.099
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
An anthocyanin-rich extract, generated from acai (AEA), was investigated for its antioxidant properties and antiproliferative activity against C-6 rat brain glioma cells and MDA-468 human breast cancer cells. AEA has an ORAC value of 2589 mu moles trolox equivalents (TE)/g dried powder and a DPPH radical-scavenging activity of 1208 mu moles TE/g, suggesting that AEA is an exceptional source of natural antioxidants. In addition, AEA remarkably suppresses proliferation of C-6 rat brain glioma cells, but has no effect on the growth of MDA-468 human breast cancer cells. Further experiments demonstrated that the AEA treatment dose-dependently inhibited the growth of C-6 rat glioma cells with an IC50 of 121 mu g/ml. The DNA ladder fragmentation results indicated that AEA induced apoptosis of C-6 rat brain glioma cells. To compare acai with other anthocyanin-rich extracts, a number of berry extracts, including blueberry, strawberry, raspberry, blackberry and wolfberry, were assessed for potential antiproliferative activity against C-6 rat brain glioma cells. However, none of them showed suppressing effect. The results suggest that the active antiproliferative constituents in AEA are unlikely to be anthocyanins normally found in common berries. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:208 / 214
页数:7
相关论文
共 37 条
[1]  
Balasundram N, 2003, ASIA PACIFIC J CLI S, V12, pS37
[2]   Fruits and vegetables and endometrial cancer risk: A systematic literature review and meta-analysis [J].
Bandera, Elisa V. ;
Kushi, Lawrence H. ;
Moore, Dirk F. ;
Gifkins, Dina M. ;
McCullough, Marjorie L. .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2007, 58 (01) :6-21
[3]   Stability and stabilization of the anthocyanins from Euterpe oleracea Mart [J].
Bobbio, FO ;
Bobbio, PA ;
Oliveira, PA ;
Fadelli, S .
ACTA ALIMENTARIA, 2002, 31 (04) :371-377
[4]   Quercetin inhibition of ROS-dependent and -independent apoptosis in rat glioma C6 cells [J].
Chen, Tong-Jong ;
Jeng, Jing-Yueh ;
Lin, Cheng-Wei ;
Wu, Chin-Yen ;
Chen, Yen-Chou .
TOXICOLOGY, 2006, 223 (1-2) :113-126
[5]   High-throughput relative DPPH radical scavenging capacity assay [J].
Cheng, Zhihong ;
Moore, Jeffrey ;
Yu, Liangli .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (20) :7429-7436
[6]   Acai (Euterpe oleracea mart.) polyphenolics in their glycoside and aglycone forms induce apoptosis of HL-60 leukemia cells [J].
Del Pozo-Insfran, D ;
Percival, SS ;
Talcott, ST .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (04) :1222-1229
[7]   Antioxidant properties of prepared blueberry (Vaccinium myrtillus) extracts [J].
Faria, A ;
Oliveira, J ;
Neves, P ;
Gameiro, P ;
Santos-Buelga, C ;
de Freitas, V ;
Mateus, N .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (17) :6896-6902
[8]   Generation of superoxide by the mitochondrial Complex I [J].
Grivennikova, Vera G. ;
Vinogradov, Andrei D. .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2006, 1757 (5-6) :553-561
[9]   Antioxidant activity of dietary fruits, vegetables, and commercial frozen fruit pulps [J].
Hassimotto, NMA ;
Genovese, MI ;
Lajolo, FM .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (08) :2928-2935
[10]   Proanthocyanidin profile and ORAC values of manitoba berries, chokecherries, and seabuckthorn [J].
Hosseinian, Farah S. ;
Li, Wende ;
Hydamaka, Arnie W. ;
Tsopmo, Apollinaire ;
Lowry, Lynda ;
Friel, James ;
Beta, Trust .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (17) :6970-6976