ATP receptors and giant cell formation

被引：41

作者：

Di Virgilio, F

Falzoni, S

Chiozzi, P

Sanz, JM

Ferrari, D

Buell, CN

机构：

[1] Univ Ferrara, Dept Expt & Diagnost Med, Sect Gen Pathol, I-44100 Ferrara, Italy

[2] Univ Ferrara, Ctr Biotechnol, I-44100 Ferrara, Italy

[3] Serono Pharmaceut Res Inst, Geneva, Switzerland

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1999年 / 66卷 / 05期

关键词：

P2 purinergic receptor; chronic inflammation; granuloma;

D O I：

10.1002/jlb.66.5.723

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have investigated the role of the purinergic P2X(7) receptor in the formation of multinucleated giant cells in human monocyte/macrophage cultures stimulated with either concanavalin A or phytohemagglutinin. Macrophage fusion can be blocked by a P2X(7)-selective pharmacological antagonist or by a mAb directed against the extracellular P2X(7) domain, Furthermore, macrophage cell, clones expressing high P2X(7) levels spontaneously fuse in culture, whereas macrophage clones lacking P2X(7) are unable to fuse, Our findings suggest that the newly identified purinergic P2X(7) receptor plays a central role in the complex chain of events leading to generation of macrophage-derived giant cells.

引用

页码：723 / 726

页数：4

共 20 条

[1] An ATP-activated channel is involved in mitogenic stimulation of human T lymphocytes
Baricordi, OR
Ferrari, D
Melchiorri, L
Chiozzi, P
Hanau, S
Chiari, E
Rubini, M
DiVirgilio, F
[J]. BLOOD, 1996, 87 (02) : 682 - 690
[2] Blockade of human P2X7 receptor function with a monoclonal antibody
Buell, G
Chessell, IP
Michel, AD
Colo, G
Salazzo, M
Herren, S
Gretener, D
Grahames, C
Kaur, R
Kosco-Vilbois, MH
Humphrey, PPA
[J]. BLOOD, 1998, 92 (10) : 3521 - 3528
[3] Buell GN, 1998, RECEPTOR CHANNEL, V5, P347
[4] Spontaneous cell fusion in macrophage cultures expressing high levels of the P2Z/P2X(7) receptor
Chiozzi, P
Sanz, JM
Ferrari, D
Falzoni, S
Aleotti, A
Buell, GN
Collo, G
DiVirgilio, F
[J]. JOURNAL OF CELL BIOLOGY, 1997, 138 (03) : 697 - 706
[5] Role of the purinergic P2Z receptor in spontaneous cell death in J774 macrophage cultures
Chiozzi, P
Murgia, M
Falzoni, S
Ferrari, D
DiVirgilio, F
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (01) : 176 - 181
[6] Cotran RS, 1994, PATHOLOGIC BASIS DIS, P51
[7] Di Virgilio F, 1998, DRUG DEVELOP RES, V45, P207, DOI 10.1002/(SICI)1098-2299(199811/12)45:3/4<207::AID-DDR18>3.0.CO
[8] 2-N
[9] EXTRACELLULAR ATP AS A POSSIBLE MEDIATOR OF CELL-MEDIATED CYTOTOXICITY
DIVIRGILIO, F
PIZZO, P
ZANOVELLO, P
BRONTE, V
COLLAVO, D
[J]. IMMUNOLOGY TODAY, 1990, 11 (08): : 274 - 277
[10] THE PURINERGIC P-2Z RECEPTOR OF HUMAN MACROPHAGE CELLS - CHARACTERIZATION AND POSSIBLE PHYSIOLOGICAL-ROLE
FALZONI, S
MUNERATI, M
FERRARI, D
SPISANI, S
MORETTI, S
DIVIRGILIO, F
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (03) : 1207 - 1216

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