A short-chain peptide toxin isolated from Centruroides sculpturatus scorpion venom inhibits ether-a-go-go-related gene K+ channels

被引：31

作者：

Nastainczyk, W

Meves, H ^{[1
]}

Watt, DD

机构：

[1] Univ Saarland, Dept Physiol, D-66421 Homburg, Germany

[2] Univ Saarland, Dept Biochem, D-66421 Homburg, Germany

[3] Creighton Univ, Coll Med, Omaha, NE 68178 USA

来源：

TOXICON | 2002年 / 40卷 / 07期

关键词：

ether-a-go-go-related gene K+ channels; scorpion toxin; ergtoxin; CsEKerg1;

D O I：

10.1016/S0041-0101(02)00100-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

From the venom of the American scorpion Centruroides sculpturatus Ewing we have isolated a minute peptide fraction (named CsEKerg 1) which reversibly inhibits the current through ERG (ether-a-go-go-related gene) K+ channels. Isolation was done by CM-cellulose column chromatography and reversed phase high-performance liquid chromatography. To test for an effect on ERG channels we used NG108-15 neuroblastoma X glioma hybrid cells voltage-clamped in the whole-cell mode. CsEKerg I contains 43 amino acids and has a molecular weight of 4833. Its amino acid sequence is similar but not identical to that of ergtoxin, a peptide isolated recently from the venom of the Mexican scorpion Centruroides noxius [FASEB J. 13 (1999) 953]. Half inhibition of ERG current occurs at a peptide concentration of 1.12 mug/ml. (C) 2002 Elsevier Science Ltd. All rights reserved.

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页码：1053 / 1058

页数：6

相关论文

共 15 条

[1] A novel inward-rectifying K+ current with a cell-cycle dependence governs the resting potential of mammalian neuroblastoma cells [J].

Arcangeli, A ;

Bianchi, L ;

Becchetti, A ;

Faravelli, L ;

Coronnello, M ;

Mini, E ;

Olivotto, M ;

Wanke, E .

JOURNAL OF PHYSIOLOGY-LONDON, 1995, 489 (02) :455-471

[2] AMINO-ACID SEQUENCES OF NEUROTOXIC PROTEIN VARIANTS FROM VENOM OF CENTRUROIDES-SCULPTURATUS-EWING [J].

BABIN, DR ;

WATT, DD ;

GOOS, SM ;

MLEJNEK, RV .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1974, 164 (02) :694-706

[3] AMINO-ACID SEQUENCE OF NEUROTOXIN-I FROM CENTRUROIDES-SCULPTURATUS EWING [J].

BABIN, DR ;

WATT, DD ;

GOOS, SM ;

MLEJNEK, RV .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 166 (01) :125-134

[4] The erg inwardly rectifying K+ current and its modulation by thyrotrophin-releasing hormone in giant clonal rat anterior pituitary [J].

Bauer, CK .

JOURNAL OF PHYSIOLOGY-LONDON, 1998, 510 (01) :63-70

[5] Disulfide bridges of Ergtoxin, a member of a new sub-family of peptide blockers of the ether-a-go-go-related K+ channel [J].

Bottiglieri, C ;

Ferrara, L ;

Corona, M ;

Gurrola, GB ;

Batista, C ;

Wanke, E ;

Possani, LD .

FEBS LETTERS, 2000, 479 (03) :156-157

[6] MODIFICATION OF SODIUM CHANNEL GATING IN FROG MYELINATED NERVE-FIBERS BY CENTRUROIDES-SCULPTURATUS SCORPION-VENOM [J].

CAHALAN, MD .

JOURNAL OF PHYSIOLOGY-LONDON, 1975, 244 (02) :511-534

[7] Genes and peptides from the scorpion Centruroides sculpturatus Ewing, that recognize Na+-channels [J].

Corona, M ;

Valdez-Cruz, NA ;

Merino, E ;

Zurita, M ;

Possani, LD .

TOXICON, 2001, 39 (12) :1893-1898

[8] A toxin to nervous, cardiac, and endocrine ERG K+ channels isolated from Centruroides noxius scorpion venom [J].

Gurrola, GB ;

Rosati, B ;

Rocchetti, M ;

Pimienta, G ;

Zaza, A ;

Arcangeli, A ;

Olivotto, M ;

Possani, LD ;

Wanke, E .

FASEB JOURNAL, 1999, 13 (08) :953-962

[9] An ERG channel inhibitor from the scorpion Buthus eupeus [J].

Korolkova, YV ;

Kozlov, SA ;

Lipkin, AV ;

Pluzhnikov, KA ;

Hadley, JK ;

Filippov, AK ;

Brown, DA ;

Angelo, K ;

Strobaek, D ;

Jespersen, T ;

Olesen, SP ;

Jensen, BS ;

Grishin, EV .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (13) :9868-9876

[10] INTERACTIONS OF SCORPION TOXINS WITH THE SODIUM-CHANNEL [J].

MEVES, H ;

SIMARD, JM ;

WATT, DD .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1986, 479 :113-132