共 19 条
Characterization of regulatory T cells identified as CD4+CD25highCD39+ in patients with active tuberculosis
被引:63
作者:
Chiacchio, T.
[1
]
Casetti, R.
[2
]
Butera, O.
[1
]
Vanini, V.
[1
]
Carrara, S.
[1
]
Girardi, E.
[1
]
Di Mitri, D.
[3
]
Battistini, L.
[3
]
Martini, F.
[2
]
Borsellino, G.
[3
]
Goletti, D.
[1
]
机构:
[1] Ist Nazl Malattie Infett L Spallanzani, IRCCS, Translat Res Unit, I-00149 Rome, Italy
[2] Ist Nazl Malattie Infett L Spallanzani, IRCCS, Cellular Immunol Lab, Dept Epidemiol & Preclin Res, I-00149 Rome, Italy
[3] Santa Lucia Fdn, Lab Neuroimmunol, Rome, Italy
关键词:
CD39;
FoxP3;
RD1;
proteins;
TB;
T-reg;
EXPRESSION;
DISEASE;
STIMULATION;
SUPPRESSION;
ANTIGENS;
IMMUNITY;
BLOOD;
D O I:
10.1111/j.1365-2249.2009.03908.x
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
Forkhead box P3 (FoxP3) is a transcription factor whose expression characterizes regulatory T cells (T-reg), but it is also present on activated T cells, thus hindering correct T-reg identification. Using classical markers for T-reg recognition, discordant results were found in terms of T-reg expansion during active tuberculosis (TB) disease. Recently CD39 has been shown to be an accurate marker for T-reg detection. The objectives of this study were: (i) to identify T-reg expressing CD39 in patients with TB and to compare the results with those obtained by the standard phenotypic markers; (ii) to evaluate if T-reg are expanded in vitro by exogenous interleukin (IL)-2 or by antigen-specific stimulation; and (iii) to characterize T-reg function on the modulation of antigen-specific responses. We enrolled 13 patients with pulmonary TB and 12 healthy controls. T-reg were evaluated by flow cytometry ex vivo and after antigen-specific in vitro stimulation using CD25, FoxP3, CD127 and CD39 markers. Results indicate that CD39(+) cells within the CD4(+)CD25(high) cells have T-reg properties (absence of interferon-gamma production and transforming growth factor-beta 1 release upon stimulation). Ex vivo analysis did not show significant differences between TB patients and controls of T-reg by classical or novel markers. In contrast, a significantly higher percentage of T-reg was found in TB patients after antigen-specific stimulation both in the presence or absence of IL-2. Depletion of CD39(+) T-reg increased RD1-specific responses significantly. In conclusion, CD39 is an appropriate marker for T-reg identification in TB. These results can be useful for future studies to monitor Mycobacterium tuberculosis-specific response during TB.
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页码:463 / 470
页数:8
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