Primary sequence of the human, lysine-rich, ribosomal protein RPL38 and detection of an unusual RPL38, processed pseudogene in the promoter region of the type-1 angiotensin II receptor gene

被引:12
作者
Espinosa, L [1 ]
Martin, M [1 ]
Nicolas, A [1 ]
Fabre, M [1 ]
Navarro, E [1 ]
机构
[1] UNIV AUTONOMA BARCELONA,INST MUNICIPAL INVEST MED,UBCM,E-08003 BARCELONA,SPAIN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1354卷 / 01期
关键词
HSRPL38; ribosomal protein; processed pseudogene; human type-1 angiotensin II receptor gene;
D O I
10.1016/S0167-4781(97)00124-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated a cDNA clone encoding the human ribosomal protein L38 (HSRPL38). The longest ORF of the cDNA predicts a lysine-rich small polypeptide identical to the rat RPL38 protein (100% identity), and sharing a 84% of identity to the tomato RPL38 protein sequence. Northern blot analysis of a number of epithelial cell lines showed that the HSRPL38 is encoded by a mRNA ubiquitously expressed. Southern blot analysis of human genomic DNA suggested that the RPL38 does not constitute a multigene family but it is encoded by a reduced set of active genes, among which we have also found a RPL38 processed pseudogene located in the promoter region of the human type-1 angiotensin II receptor gene. This RPL38 pseudogene is very unusual among processed pseudogenes in that the poly A tail and the entire 5'-UTR of the original RPL38 mRNA were deleted during the retrotransposition process. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:58 / 64
页数:7
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