Saussurea tridactyla Sch Bip.-derived polysaccharides and flavones reduce oxidative damage in ultraviolet B-irradiated HaCaT cells via a p38MAPK-independent mechanism

被引：8

作者：

Guo, Yan ^{[1
]}

Sun, Juan ^{[2
]}

Ye, Juan ^{[1
]}

Ma, Wenyu ^{[1
]}

Yan, Hualing ^{[1
]}

Wang, Gang ^{[1
]}

机构：

[1] Qinghai Univ, Affiliated Hosp, Dept Dermatol & Venereol, 29 Tongren Rd, Xining 810001, Qinghai, Peoples R China

[2] Qinghai Univ, Coll Med, Dept Anat, Xining 810001, Qinghai, Peoples R China

来源：

DRUG DESIGN DEVELOPMENT AND THERAPY | 2016年 / 10卷

关键词：

Saussurea tridactyla Sch Bip.-derived polysaccharides; flavones; oxidative damage; p38MAPK-independent mechanism; HUMAN SKIN KERATINOCYTES; ENDOPLASMIC-RETICULUM STRESS; UVB RADIATION; MOUSE SKIN; APOPTOSIS; PROTECTS; FULLERENE-C60; GENERATION; HERB;

D O I：

10.2147/DDDT.S96581

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Objective: To investigate whether Saussurea tridactyla Sch. Bip.-derived polysaccharides and flavones exert apoptosis-inhibiting effects in ultraviolet B (UVB)-irradiated HaCaT cells. Methods: We divided HaCaT cells into low radiation UVB and high radiation UVB groups. Low radiation UVB and high radiation UVB groups were further divided into a control group, UVB radiation group (UVB group), S. tridactyla Sch. Bip.-derived polysaccharides and flavones low-dose group, and S. tridactyla Sch. Bip.-derived polysaccharides and flavones high-dose group. Cell viability and morphology were assayed by MTT and trypan blue staining. Superoxide dismutase activity, glutathione content, malondialdehyde content, and catalase activity test kits were used to detect superoxide dismutase activity, glutathione content, malondialdehyde content, and catalase activity, respectively. Cell apoptosis, intracellular Ca2+ levels, and mitochondrial membrane potential (Delta psi) were detected by flow cytometry. Protein levels were analyzed by Western blotting and immunofluorescence. Results: S. tridactyla Sch. Bip.-derived polysaccharides and flavones were found to increase the absorbance of MTT, decrease cell death, alleviate the degree of cell edema, restore the cell morphology, reduce cell death fragments and chip phenomenon, increase superoxide dismutase activity, glutathione content, and catalase activity while decreasing the content of malondialdehyde, lowering the population of apoptotic cells, reducing the intracellular Ca2+ fluorescence, increasing the mitochondrial membrane potential (Delta psi), increasing the expressions of p-38, p-53, Bcl-2, and decreasing the expressions of Bax and active-caspase-3. Conclusion: S. tridactyla Sch. Bip.-derived polysaccharides and flavones can reduce cell apoptosis to protect HaCaT cells from oxidative damage after UVB irradiation; however, this effect does not occur via the p38MAPK pathway.

引用

页码：389 / 403

页数：15

共 41 条

[1]

Delphinidin, an anthocyanidin in pigmented fruits and vegetables, protects human HaCaT keratinocytes and mouse skin against UVB-mediated oxidative stress and apoptosis [J].