The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA

被引：8

作者：

Barbosa, GHTS

Santana, EM

Almeida, AMP

Araujo, AM

Fatibello, O

Carvalho, LB ^{[1
]}

机构：

[1] UFPE, Lab Imunopatol Keizo Asami, BR-50670420 Recife, PE, Brazil

[2] UFPE, Dept Trop Med, BR-50670420 Recife, PE, Brazil

[3] UFPE, Dept Bioquim, BR-50670420 Recife, PE, Brazil

[4] FIOCRUZ, Ctr Pesquisas Aggeu Magalhaes, Recife, PE, Brazil

[5] Fdn Hematol & Hemoterapia Pernambuco, Recife, PE, Brazil

[6] Univ Fed Sao Carlos, Dept Quim, Sao Carlos, SP, Brazil

来源：

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2000年 / 33卷 / 07期

关键词：

filter paper; polyvinyl alcohol; glutaraldehyde; ELISA; dot-ELISA; plague;

D O I：

10.1590/S0100-879X2000000700013

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 mu g of Fl antigen/disc, 3% w/v skim milk in PSS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this method economically attractive.

引用

页码：823 / 827

页数：5

共 19 条

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