Evaluation of MicroScan Autoscan for identification of Pseudomonas aeruginosa isolates from cystic fibrosis patients

被引:21
作者
Saiman, L
Burns, JL
Larone, D
Chen, YH
Garber, E
Whittier, S
机构
[1] Columbia Univ, Div Infect Dis, Dept Pediat, New York, NY 10032 USA
[2] New York Presbyterian Hosp, Columbia Presbyterian Ctr, Dept Pathol, New York, NY USA
[3] Univ Washington, Dept Pediat, Div Infect Dis, Childrens Hosp, Seattle, WA USA
[4] Univ Washington, Reg Med Ctr, Seattle, WA USA
关键词
D O I
10.1128/JCM.41.1.492-494.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Accurate identification of gram-negative bacilli from cystic fibrosis (CF) patients is essential. Only 57% (108 of 189) of nonmucoid strains and 40% (24 of 60) of mucoid strains were definitively identified as Pseudomonas aeruginosa with MicroScan Autoscan. Most common misidentifications were Pseudomonas fluorescens-Pseudomonas putida (i.e., the strain was either P. fluorescens or P. putida, but the system did not make the distinction and yielded the result P. fluorescens/putida) and Alcaligenes spp. Extending the incubation to 48 h improved identification, but 15% of isolates remained misidentified. The MicroScan Autoscan system cannot be recommended for the identification of P. aeruginosa isolates from CF patients.
引用
收藏
页码:492 / 494
页数:3
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