Host sequences flanking the human T-cell leukemia virus type 1 provirus in vivo

被引:36
作者
Leclercq, I
Mortreux, F
Cavrois, M
Leroy, A
Gessain, A
Wain-Hobson, S
Wattel, E
机构
[1] Univ Lyon 1, Ctr Leon Berard, Unite Oncogenese Virale, CNRS,UMR 5537, F-69373 Lyon 08, France
[2] Inst Rech Canc Lille, INSERM, U524, Lille, France
[3] Ctr Oscar Lambret, Unite Oncogenese Virale, F-59020 Lille, France
[4] Inst Pasteur, Unite Epidemiol Virus Oncogenes, Paris, France
[5] Inst Pasteur, Unite Retrovirol Mol, Paris, France
[6] CHU Lille, Serv Malad Sang, F-59037 Lille, France
关键词
D O I
10.1128/JVI.74.5.2305-2312.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human pathogenic retroviruses do not have common: loci of: integration. However, many factors, such as chromatin structure, transcriptional activity, DNA-protein interaction, CpG methylation, and nucleotide composition of the target sequence, may influence integration site selection. These features have been investigated by in vitro integration reactions or by infection of cell lines with recombinant retroviruses. Less is known about target choice for integration in vivo. The present study was conducted in order to assess the characteristics of cellular sequences targeted for human T-cell leukemia virus type 1 (HTLV-1) integration in vivo. Sequencing integration sites from greater than or equal to 200 proviruses (19 kb of sequence): isolated from 29 infected individuals revealed that HTLV-1 integration is not random at the level of the nucleotide sequence. The virus was found to integrate in A/T-rich regions with a weak consensus sequence at positions within and without of the hexameric repeat generated during integration. These features were not associated with a preference for integration near active regions or repeat elements of the host chromosomes. Most or all of the regions of the genome appear to be accessible to HTLV-1 integration. As with integration in vitro, integration specificity in vivo seems to be determined by local features rather than by the accessibility of specific regions.
引用
收藏
页码:2305 / 2312
页数:8
相关论文
共 53 条
[31]   HUMAN-IMMUNODEFICIENCY-VIRUS INTEGRASE DIRECTS INTEGRATION TO SITES OF SEVERE DNA DISTORTION WITHIN THE NUCLEOSOME CORE [J].
PRUSS, D ;
BUSHMAN, FD ;
WOLFFE, AP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :5913-5917
[32]   NUCLEOSOMES, DNA-BINDING PROTEINS, AND DNA-SEQUENCE MODULATE RETROVIRAL INTEGRATION TARGET SITE SELECTION [J].
PRYCIAK, PM ;
VARMUS, HE .
CELL, 1992, 69 (05) :769-780
[33]   PATTERNS OF PROVIRAL INSERTION AND DELETION IN AVIAN-LEUKOSIS VIRUS-INDUCED LYMPHOMAS [J].
ROBINSON, HL ;
GAGNON, GC .
JOURNAL OF VIROLOGY, 1986, 57 (01) :28-36
[34]   RETROVIRUS INTEGRATION AND CHROMATIN STRUCTURE - MOLONEY MURINE LEUKEMIA PROVIRAL INTEGRATION SITES MAP NEAR DNASE I-HYPERSENSITIVE SITES [J].
ROHDEWOHLD, H ;
WEIHER, H ;
REIK, W ;
JAENISCH, R ;
BREINDL, M .
JOURNAL OF VIROLOGY, 1987, 61 (02) :336-343
[35]   THE ISOPYCNIC, COMPARTMENTALIZED INTEGRATION OF ROUS-SARCOMA VIRUS SEQUENCES [J].
RYNDITCH, A ;
KADI, F ;
GERYK, J ;
ZOUBAK, S ;
SVOBODA, J ;
BERNARDI, G .
GENE, 1991, 106 (02) :165-172
[36]   NONRANDOM DISTRIBUTION OF MMTV PROVIRAL SEQUENCES IN THE MOUSE GENOME [J].
SALINAS, J ;
ZERIAL, M ;
FILIPSKI, J ;
CREPIN, M ;
BERNARDI, G .
NUCLEIC ACIDS RESEARCH, 1987, 15 (07) :3009-3022
[37]  
SANDMEYER SB, 1990, ANNU REV GENET, V24, P491
[38]   TRANSCRIPTIONALLY ACTIVE GENOME REGIONS ARE PREFERRED TARGETS FOR RETROVIRUS INTEGRATION [J].
SCHERDIN, U ;
RHODES, K ;
BREINDL, M .
JOURNAL OF VIROLOGY, 1990, 64 (02) :907-912
[39]   NONSPECIFIC INTEGRATION OF THE HTLV PROVIRUS GENOME INTO ADULT T-CELL LEUKEMIA-CELLS [J].
SEIKI, M ;
EDDY, R ;
SHOWS, TB ;
YOSHIDA, M .
NATURE, 1984, 309 (5969) :640-642
[40]   HIGHLY PREFERRED TARGETS FOR RETROVIRUS INTEGRATION [J].
SHIH, CC ;
STOYE, JP ;
COFFIN, JM .
CELL, 1988, 53 (04) :531-537